Mouse CASP14 (Caspase 14) CLIA Kit (E-CL-M0178)

Name: Mouse CASP14 (Caspase 14) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0178
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse CASP14 in samples.No significant cross-reactivity or interference between Mouse CASP14 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	61701.000	62035.000	61868.000	61838
2000	28773.000	29525.000	29149.000	29119
1000	14744.000	14218.000	14481.000	14451
500	6846.000	7024.000	6935.000	6905
250	3592.000	3062.000	3327.000	3297
125	1768.000	1522.000	1645.000	1615
62.5	768.000	842.000	805.000	775
0	30.000	30.000	30.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	205.620	356.970	1501.280	212.830	379.640	1527.760
Standard deviation	26.710	41.840	125.960	26.540	43.810	158.280
CV（%）	12.990	11.720	8.390	12.470	11.540	10.360

Recovery

The recovery of Mouse CASP14 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	88-102	93
EDTA plasma （n=8）	92-102	97
Cell culture media （n=8）	94-107	99

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse CASP14 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	89-101	96-113	87-102
1:2	Average	94	104	94
1:4	Range	102-115	90-103	88-104
1:4	Average	109	96	95
1:8	Range	90-104	88-102	101-113
1:8	Average	97	93	106

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CASP14. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CASP14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CASP14, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse CASP14. You can calculate the concentration of Mouse CASP14 in the samples by comparing the RLU value of the samples to the standard curve.

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