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Mouse CASP3(Caspase 3) ELISA Kit

Citations(10)Uniprot : P70677

Cat.No.:E-EL-M0238
Reactivity: Mouse

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96T
48T
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96T*5
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse CASP3 in samples. No significant cross-reactivity or interference between Mouse CASP3 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse CASP3 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CASP3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CASP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CASP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CASP3. You can calculate the concentration of Mouse CASP3 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse CASP3 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse CASP3 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.53	1.29	4.47	0.50	1.40	4.20
Standard deviation	0.03	0.06	0.19	0.03	0.06	0.23
CV (%)	5.66	4.65	4.25	6.00	4.29	5.48

Recovery

The recovery of Mouse CASP3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-97	92
EDTA plasma (n=8)	91-105	96
Cell culture media (n=8)	85-101	92

Linearity

Samples were spiked with high concentrations of Mouse CASP3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P70677
Synonyms	CPP32, CPP32B, SCA-1, Apoptain, Yama
Research Area	Cancer, Cell Biology, Metabolism, Neuroscience

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

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Citations

BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
Curcumin modulates multiple cell death, matrix metalloproteinase activation and cardiac protein release in susceptible and resistant Plasmodium berghei-infected mice

DOI: 10.1016/j.biopha.2021.112454

PMID: 34894518
Sample: serum
NEUROTOXICOLOGY (2021) IF: 4.294
IDO-1 inhibition protects against neuroinflammation, oxidative stress and mitochondrial dysfunction in 6-OHDA induced murine model of Parkinson’s disease

DOI: 10.1016/j.neuro.2021.03.009

Sample: Plasma,Tissue homogenate(Brain)
INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH (2022) IF: 4.477
Potential Protective Effects of Ruta Chalepensis L. Extracts Against Gentamicin-Induced Nephrotoxicity via Reduction on Apoptotic, DNA Damage and Oxidative Stress Markers in Mice

DOI: 10.1080/09603123.2022.2105824

PMID: 35917490
Sample: kidney tissue
Frontiers in Physiology (2020) IF: 3.367
Effects of Human Umbilical Cord-Derived Mesenchymal Stem Cells on the Acute Cigarette Smoke-Induced Pulmonary Inflammation Model

DOI: 10.3389/fphys.2020.00962

PMID: 32903481
Sample: Tissue homogenate
Brain and Behavior (2022) IF: 3.405
The protective effects of nesfatin-1 in neurological dysfunction after spinal cord injury by inhibiting neuroinflammation

DOI: 10.1002/brb3.2778

PMID: 36271663
Sample: Spinal cord injury (SCI) area
Journal of Personalized Medicine (2022) IF: 3.508
Artichoke (Cynara Scolymus) Methanolic Leaf Extract Alleviates Diethylnitrosamine-Induced Toxicity in BALB/c Mouse Brain: Involvement of Oxidative Stress and Apoptotically Related Klotho/PPARγ Signaling

DOI: 10.3390/jpm12122012

Sample: brain
NEUROSCIENCE (2022) IF: 3.59
Pharmacological Postconditioning by Protocatechuic Acid Attenuates Brain Injury in Ischemia–Reperfusion (I/R) Mice Model: Implications of Nuclear Factor Erythroid-2-Related Factor Pathway

DOI: 10.1016/j.neuroscience.2022.03.016

Sample: brain
HUMAN & EXPERIMENTAL TOXICOLOGY (2017) IF: 1.802
6-Gingerol improves testicular function in mice model of chronic ulcerative colitis:

DOI: 10.1177/0960327117703689

Sample: testes
Toxicology Reports (2022)
Hexane fraction of Globimetula braunii induces mitochondria-mediated apoptosis in Plasmodium berghei-infected mice

DOI: 10.1016/j.toxrep.2022.04.002

Sample: liver tissue
Journal of Essential Oil Bearing Plants (2019) IF: 0.6879
The Protective Effects of Thymol Against Ketoprofen Induced Damages on Pancreatic Acinar and Islet of Langerhans Cells in Rats

DOI: 10.1080/0972060X.2019.1625814

Sample: Tissue homogenate

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