Mouse CKM (Creatine Kinase, Muscle) CLIA Kit (E-CL-M0231)

Name: Mouse CKM (Creatine Kinase, Muscle) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0231
Price: 180.0 USD
Availability: InStock

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Mouse CKM (Creatine Kinase, Muscle) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.19 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse CKM in samples.No significant cross-reactivity or interference between Mouse CKM and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
20	34144.000	37436.000	35790.000	35761
10	13026.000	13188.000	13107.000	13078
5	5922.000	5390.000	5656.000	5627
2.5	2686.000	2846.000	2766.000	2737
1.25	1561.000	1413.000	1487.000	1458
0.63	938.000	912.000	925.000	896
0.31	630.000	676.000	653.000	624
0	28.000	30.000	29.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	1.030	3.050	9.610	1.070	2.750	10.300
Standard deviation	0.110	0.250	0.860	0.120	0.220	0.880
CV（%）	10.680	8.200	8.950	11.210	8.000	8.540

Recovery

The recovery of Mouse CKM was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	87-100	93
EDTA plasma （n=8）	88-100	93
Cell culture media （n=8）	97-114	104

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse CKM and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	91-103	99-114	98-115
1:2	Average	97	105	106
1:4	Range	92-104	96-112	83-95
1:4	Average	98	103	90
1:8	Range	88-102	91-104	88-100
1:8	Average	95	98	93

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CKM. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CKM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CKM, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse CKM. You can calculate the concentration of Mouse CKM in the samples by comparing the RLU value of the samples to the standard curve.

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