Mouse CNTF (Ciliary Neurotrophic Factor) CLIA Kit (E-CL-M0195)

Name: Mouse CNTF (Ciliary Neurotrophic Factor) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0195
Price: 180.0 USD
Availability: InStock

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Mouse CNTF (Ciliary Neurotrophic Factor) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse CNTF in samples.No significant cross-reactivity or interference between Mouse CNTF and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	49568.000	58286.000	53927.000	53888
400	22391.000	25255.000	23823.000	23784
200	11201.000	9669.000	10435.000	10396
100	4300.000	4894.000	4597.000	4558
50	2488.000	2128.000	2308.000	2269
25	1102.000	1038.000	1070.000	1031
12.5	491.000	551.000	521.000	482
0	37.000	41.000	39.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	43.840	99.640	320.260	40.530	101.450	341.540
Standard deviation	4.180	9.420	32.190	3.450	10.870	21.820
CV（%）	9.530	9.450	10.050	8.510	10.710	6.390

Recovery

The recovery of Mouse CNTF was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-105	97
EDTA plasma （n=8）	96-112	103
Cell culture media （n=8）	97-108	102

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse CNTF and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	100-116	95-112	95-111
1:2	Average	106	103	103
1:4	Range	97-113	89-99	102-118
1:4	Average	104	94	110
1:8	Range	93-108	85-97	98-114
1:8	Average	100	90	105

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CNTF. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CNTF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CNTF, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse CNTF. You can calculate the concentration of Mouse CNTF in the samples by comparing the RLU value of the samples to the standard curve.

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