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Mouse COL1(Collagen Type Ⅰ) ELISA Kit

Cat.No.:E-EL-M0325
Reactivity: Mouse

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96T
48T
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96T*5
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.31-20 ng/mL
Sensitivity	0.19 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse COL1 in samples. No significant cross-reactivity or interference between Mouse COL1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse COL1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse COL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse COL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse COL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse COL1. You can calculate the concentration of Mouse COL1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse COL1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse COL1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	1.02	2.49	9.30	1.12	2.27	10.16
Standard deviation	0.05	0.13	0.28	0.06	0.12	0.42
CV (%)	4.90	5.22	3.01	5.36	5.29	4.13

Recovery

The recovery of Mouse COL1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	89-102	96
EDTA plasma (n=8)	85-100	91
Cell culture media (n=8)	93-109	101

Linearity

Samples were spiked with high concentrations of Mouse COL1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	COL1
Research Area	Signal Transduction,Stem Cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Frontiers in Immunology (2020) IF: 5.085
Epithelial-Mesenchymal Transition in Asthma Airway Remodeling Is Regulated by the IL-33/CD146 Axis

DOI: 10.3389/fimmu.2020.01598

PMID: 32793232
Sample: Tissue homogenate
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020) IF: 5.162
Insight into delivery of dermal fibroblast by non-biodegradable bacterial nanocellulose composite hydrogel on wound healing

DOI: 10.1016/j.ijbiomac.2020.05.011

Sample: skin
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2021) IF: 5.31
Metformin attenuates post‐epidural fibrosis by inhibiting the TGF‐β1/Smad3 and HMGB1/TLR4 signaling pathways

DOI: 10.1111/jcmm.16398

Sample: Cell（MCF-7 cancer cells）
Frontiers in Pharmacology (2018) IF: 5.811
A Chinese Herbal Formula Ameliorates Pulmonary Fibrosis by Inhibiting Oxidative Stress via Upregulating Nrf2

DOI: 10.3389/fphar.2018.00628

Sample: Cell culture supernatant
Tissue Engineering and Regenerative Medicine (2022) IF: 4.169
IL-33 Promotes ST2-Dependent Fibroblast Maturation via P38 and TGF-β in a Mouse Model of Epidural Fibrosis

DOI: 10.1007/s13770-021-00425-1

PMID: 35195855
Sample: spine scar
Aging-US (2020) IF: 4.831
The role of Hrd1 in ultraviolet (UV) radiation induced photoaging

DOI: 10.18632/aging.103851

PMID: 33168784
Sample: Serum
Biomed Research International (2023) IF: 3.246
Long Noncoding RNA NORAD Promotes Fracture Healing through Interacting with Osteoblast Differentiation via Targeting miR-26a

DOI: 10.1155/2023/9950037

Sample: MC3T3-E1 cell
AMERICAN JOURNAL OF CHINESE MEDICINE (2019) IF: 3.51
Salvia miltiorrhiza Restrains Reactive Oxygen Species-Associated Pulmonary Fibrosis via Targeting Nrf2-Nox4 Redox Balance

DOI: 10.1142/S0192415X19500575

Sample: lung
Parasites & Vectors (2021) IF: 3.876
In vitro and in vivo efficacy of thiacloprid against Echinococcus multilocularis

DOI: 10.1186/s13071-021-04952-7

PMID: 34488852
Sample: lymphocyte
INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED (2022) IF: 2.687
MicroRNA-455–3p promotes osteoblast differentiation via targeting HDAC2

DOI: 10.1016/j.injury.2022.08.047

Sample: MC3T3-E1 cell
Oncology Letters (2018) IF: 1.664
SMOC1 silencing suppresses the angiotensin II‑induced myocardial fibrosis of mouse myocardial fibroblasts via affecting the BMP2/Smad pathway

DOI: 10.3892/ol.2018.8989

Sample: Plasmid
Journal of Ayurveda and Integrative Medicine (2017)
Exploring molecular mechanism of bone-forming capacity of Eurycoma longifolia: Evidence of enhanced expression of bone-related biomarkers

DOI: 10.1016/j.jaim.2017.04.005

Sample: calvariae origin osteoblastic cell line subclone 4

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