Mouse COL1α2 (Collagen Type I Alpha 2) CLIA Kit (E-CL-M0215)

Name: Mouse COL1α2 (Collagen Type I Alpha 2) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0215
Price: 180.0 USD
Availability: InStock

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Mouse COL1α2 (Collagen Type I Alpha 2) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse COL1α2 in samples.No significant cross-reactivity or interference between Mouse COL1α2 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	59979.000	61387.000	60683.000	60647
1000	25240.000	26536.000	25888.000	25852
500	11854.000	10432.000	11143.000	11107
250	5687.000	5815.000	5751.000	5715
125	2834.000	2640.000	2737.000	2701
62.5	1392.000	1340.000	1366.000	1330
31.25	654.000	708.000	681.000	645
0	34.000	38.000	36.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	98.230	260.000	678.630	90.990	248.330	730.760
Standard deviation	11.790	18.490	76.070	11.050	20.490	54.220
CV（%）	12.000	7.110	11.210	12.140	8.250	7.420

Recovery

The recovery of Mouse COL1α2 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	94-108	101
EDTA plasma （n=8）	99-113	106
Cell culture media （n=8）	99-111	105

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse COL1α2 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	88-100	97-111	96-110
1:2	Average	95	104	102
1:4	Range	87-101	100-116	91-103
1:4	Average	93	108	97
1:8	Range	94-107	86-99	96-108
1:8	Average	102	92	102

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse COL1α2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse COL1α2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse COL1α2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse COL1α2. You can calculate the concentration of Mouse COL1α2 in the samples by comparing the RLU value of the samples to the standard curve.

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