Mouse FE (Ferritin) CLIA Kit (E-CL-M0296)

Name: Mouse FE (Ferritin) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0296
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.19 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse FE in samples.No significant cross-reactivity or interference between Mouse FE and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
20	33870.000	40748.000	37309.000	37286
10	11765.000	14129.000	12947.000	12924
5	6167.000	5235.000	5701.000	5678
2.5	2625.000	2895.000	2760.000	2737
1.25	1456.000	1380.000	1418.000	1395
0.63	909.000	841.000	875.000	852
0.31	588.000	670.000	629.000	606
0	22.000	24.000	23.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	1.030	3.070	9.060	0.980	3.220	8.380
Standard deviation	0.090	0.290	0.600	0.120	0.310	0.530
CV（%）	8.740	9.450	6.620	12.240	9.630	6.320

Recovery

The recovery of Mouse FE was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	94-110	101
EDTA plasma （n=8）	95-110	101
Cell culture media （n=8）	100-113	107

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse FE and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	89-104	90-105	89-100
1:2	Average	95	96	94
1:4	Range	89-104	97-114	85-99
1:4	Average	96	104	91
1:8	Range	89-102	85-99	100-119
1:8	Average	94	91	108

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse FE. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FE, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse FE. You can calculate the concentration of Mouse FE in the samples by comparing the RLU value of the samples to the standard curve.

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