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Mouse FSH(Follicle Stimulating Hormone) ELISA Kit

Citations(48)

  • Cat.No.:E-EL-M0511

  • Reactivity: Mouse
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  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 1.56-100 ng/mL
Sensitivity 0.94 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse FSH in samples. No significant cross-reactivity or interference between Mouse FSH and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse FSH concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse FSH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse FSH. You can calculate the concentration of Mouse FSH in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse FSH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse FSH were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 4.70 8.90 43.10 5.10 8.40 40.20
Standard deviation 0.30 0.40 2.10 0.30 0.40 1.70
CV (%) 6.38 4.49 4.87 5.88 4.76 4.23

Recovery

The recovery of Mouse FSH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-107 98
EDTA plasma (n=8) 89-106 97
Cell culture media (n=8) 89-104 96

Linearity

Samples were spiked with high concentrations of Mouse FSH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research AreaCancer, Metabolism, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Particle and Fibre Toxicology (2022) IF: 9.4
    Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway

    DOI: 10.1186/s12989-022-00453-2

    PMID: 35177090

    		 Sample: serum
  2. ENVIRONMENTAL POLLUTION (2021) IF: 8.071
    Tannic acid repair of zearalenone-induced damage by regulating the death receptor and mitochondrial apoptosis signaling pathway in mice

    DOI: 10.1016/j.envpol.2021.117557

    PMID: 34167001

    		 Sample: serum
  3. FREE RADICAL BIOLOGY AND MEDICINE (2022) IF: 8.1010
    Inhibition of ferroptosis attenuates oligospermia in male Nrf2 knockout mice

    DOI: 10.1016/j.freeradbiomed.2022.10.314

    PMID: 36309297

    		 Sample: testicular tissues
  4. Cell Death & Disease (2021) IF: 8.4689
    MeCP2 duplication causes hyperandrogenism by upregulating LHCGR and downregulating RORα

    DOI: 10.1038/s41419-021-04277-4

    PMID: 34697294

    		 Sample: serum
  5. Frontiers in Endocrinology (2022) IF: 6.055
    Role of EGFR expressed on the granulosa cells in the pathogenesis of polycystic ovarian syndrome.

    DOI: 10.3389/fendo.2022.971564
    Sample: serum
  6. Communications Biology (2019) IF: 6.268
    In utero exposure to acetaminophen and ibuprofen leads to intergenerational accelerated reproductive aging in female mice

    DOI: 10.1038/s42003-019-0552-x
    Sample: serum
  7. METABOLISM-CLINICAL AND EXPERIMENTAL (2019) IF: 6.513
    Role of PCSK9 in lipid metabolic disorders and ovarian dysfunction in polycystic ovary syndrome

    DOI: 10.1016/j.metabol.2019.02.002

    PMID: 30768966

    		 Sample: serum
  8. ENDOCRINOLOGY (2022) IF: 5.051
    Distinctions in PCOS induced by letrozole versus dehydroepiandrosterone with high-fat diet in mouse model

    DOI: 10.1210/endocr/bqac097
    Sample: Serum
  9. Reproductive Biology and Endocrinology (2022) IF: 5.211
    Ubiquitin-like modifier 1 ligating enzyme 1 relieves cisplatin-induced premature ovarian failure by reducing endoplasmic reticulum stress in granulosa cells

    DOI: 10.1186/s12958-022-00956-9

    PMID: 35610622

    		 Sample: ovarian tissue,GCs
  10. Reproductive Biology and Endocrinology (2021) IF: 5.211
    Irisin deletion induces a decrease in growth and fertility in mice

    DOI: 10.1186/s12958-021-00702-7

    PMID: 33581723

    		 Sample: Serum
  11. Scientific Reports (2016) IF: 5.228
    Knockout of BRD7 results in impaired spermatogenesis and male infertility

    DOI: 10.1038/srep21776
    Sample: serum
  12. Food & Function (2022) IF: 5.396
    Tilapia skin peptides restore cyclophosphamide-induced premature ovarian failure via inhibiting oxidative stress and apoptosis in mice

    DOI: 10.1039/D1FO04239D

    PMID: 35083997

    		 Sample: serum
  13. Cell Death & Disease (2019) IF: 5.959
    Germline deletion of Cdyl causes teratozoospermia and progressive infertility in male mice

    DOI: 10.1038/s41419-019-1455-y

    PMID: 30850578

    		 Sample: Serum
  14. CELL AND TISSUE RESEARCH (2022) IF: 4.051
    STUB1 directs FOXQ1-mediated transactivation of Ldha gene and facilitates lactate production in mouse Sertoli cells

    DOI: 10.1007/s00441-022-03705-x
  15. Genes (2021) IF: 4.096
    DYRK1A Overexpression in Mice Downregulates the Gonadotropic Axis and Disturbs Early Stages of Spermatogenesis

    DOI: 10.3390/genes12111800

    PMID: 34828406

    		 Sample: plasma
  16. TOXICOLOGY (2021) IF: 4.221
    Environmentally relevant perinatal exposure to DBP disturbs testicular development and puberty onset in male mice

    DOI: 10.1016/j.tox.2021.152860

    PMID: 34280466

    		 Sample: Serum
  17. Scientific Reports (2017) IF: 4.259
    Diet-induced obesity impairs spermatogenesis: a potential role for autophagy

    DOI: 10.1038/srep43475
    Sample: Serum
  18. BIOLOGY OF REPRODUCTION (2021) IF: 4.285
    Chronic overload of concentration-dependent iron exerts different effects on ovarian function in C57BL/6J mice

    DOI: 10.1093/biolre/ioab040
    Sample: serum
  19. BIOLOGY OF REPRODUCTION (2022) IF: 4.285
    A decrease in cluster of differentiation 2 expression on natural killer cells is associated with polycystic ovary syndrome but not influenced by metformin in a mouse model

    DOI: 10.1093/biolre/ioac004
    Sample: serum
  20. JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
    The effect of Bu Shen Huo Xue Tang on autoimmune premature ovarian insufficiency via Modulation of the Nrf2/Keap1 signaling pathway in mice

    DOI: 10.1016/j.jep.2021.113996

    PMID: 33684516

    		 Sample: Serum
  21. MOLECULAR AND CELLULAR ENDOCRINOLOGY (2022) IF: 4.369
    CXCL13 and CXCR5 are upregulated in PCOS mice ovaries but downregulated following metformin administration

    DOI: 10.1016/j.mce.2022.111730

    PMID: 35921919

    		 Sample: serum
  22. JOURNAL OF ENDOCRINOLOGY (2019) IF: 4.381
    miR-505-3p is a repressor of puberty onset in female mice

    DOI: 10.1530/JOE-18-0533
    Sample: serum
  23. JOURNAL OF ENDOCRINOLOGY (2019) IF: 4.381
    miR-29 family regulates the puberty onset mediated by a novel Gnrh1 transcription factor TBX21

    DOI: 10.1530/JOE-19-0082
    Sample: serum
  24. Stem Cell Research & Therapy (2019) IF: 4.627
    Human amniotic mesenchymal stem cells improve the follicular microenvironment to recover ovarian function in premature ovarian failure mice

    DOI: 10.1186/s13287-019-1315-9
    Sample: serum
  25. BIOORGANIC CHEMISTRY (2020) IF: 4.831
    Two resveratrol analogs, pinosylvin and 4,4′-dihydroxystilbene, improve oligoasthenospermia in a mouse model by attenuating oxidative stress via the Nrf2-ARE pathway

    DOI: 10.1016/j.bioorg.2020.104295
    Sample: serum
  26. PLoS One (2015) IF: 3.234
    Diet-Induced Obesity in Male C57BL/6 Mice Decreases Fertility as a Consequence of Disrupted Blood-Testis Barrier

    DOI: 10.1371/journal.pone.0120775
    Sample: Serum
  27. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2022) IF: 3.322
    Rescue of obesity-induced infertility in female mice by silencing AgRP neurons

    DOI: 10.1016/j.bbrc.2022.07.032

    PMID: 35870259

    		 Sample: blood
  28. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2023) IF: 3.322
    Prepubertal exposure to copper oxide nanoparticles induces Leydig cell injury with steroidogenesis disorders in mouse testes

    DOI: 10.1016/j.bbrc.2023.02.067
    Sample: Serum
  29. Brain Sciences (2022) IF: 3.333
    Exogenous Melatonin Regulates Puberty and the Hypothalamic GnRH-GnIH System in Female Mice

    DOI: 10.3390/brainsci12111550
  30. TOXICOLOGY LETTERS (2014) IF: 3.355
    Differential proteome and gene expression reveal response to carbon ion irradiation in pubertal mice testes

    DOI: 10.1016/j.toxlet.2014.01.001
    Sample: Serum
  31. Toxicology Research (2021) IF: 3.524
    The modulatory impacts of Glycyrrhiza glabra extract against methotrexate-induced testicular dysfunction and oxidative stress

    DOI: 10.1093/toxres/tfab049
    Sample: serum
  32. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2020) IF: 3.606
    Protective roles and mechanisms of rosmarinic acid in cyclophosphamide‐induced premature ovarian failure

    DOI: 10.1002/jbt.22591
    Sample: Serum
  33. Heliyon (2020) IF: 3.776
    The intake of an extract from seeds of Tamarindus indica L. modulates the endocrine function of adult male mice under a high fat diet

    DOI: 10.1016/j.heliyon.2020.e03310

    PMID: 32051875

    		 Sample: Serum
  34. AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2023) IF: 3.777
    Hydroxychloroquine protects against autoimmune premature ovarian insufficiency by modulating the Treg/Th17 cell ratio in BALB/c mice

    DOI: 10.1111/aji.13686
    Sample: blood
  35. REPRODUCTIVE BIOMEDICINE ONLINE (2021) IF: 3.828
    Ovarian inflammatory mRNA profiles of a dehydroepiandrosterone plus high-fat diet-induced polycystic ovary syndrome mouse model

    DOI: 10.1016/j.rbmo.2021.10.024
    Sample: serum
  36. MOLECULAR AND CELLULAR ENDOCRINOLOGY (2020) IF: 3.871
    MiR-199-3p modulates the onset of puberty in rodents probably by regulating the expression of Kiss1 via the p38 MAPK pathway

    DOI: 10.1016/j.mce.2020.110994
    Sample: serum
  37. Reproductive Biology (2022) IF: 2.376
    The effect of icariin on autoimmune premature ovarian insufficiency via modulation of Nrf2/HO-1/Sirt1 pathway in mice

    DOI: 10.1016/j.repbio.2022.100638

    PMID: 35344846

    		 Sample: serum
  38. MOLECULAR REPRODUCTION AND DEVELOPMENT (2020) IF: 2.609
    Adoptive transfers of CD4+CD25+ Tregs partially alleviate mouse premature ovarian insufficiency

    DOI: 10.1002/mrd.23404
    Sample: Serum
  39. Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
    Effect of Electroacupuncture on Reproductive Disorders and Insulin Resistance in a Murine Polycystic Ovary Syndrome Model

    DOI: 10.1155/2021/9968463

    PMID: 34987599

    		 Sample: serum
  40. Reproductive Biology and Endocrinology (2018) IF: 2.852
    The preclinical evaluation of immunocontraceptive vaccines based on canine zona pellucida 3 (cZP3) in a mouse model

    DOI: 10.1186/s12958-018-0362-x
    Sample: Serum
  41. Reproductive Biology and Endocrinology (2018) IF: 2.852
    The ovarian estrogen synthesis function was impaired in Y123F mouse and partly restored by exogenous FSH supplement

    DOI: 10.1186/s12958-018-0365-7
    Sample: Serum
  42. Reproductive Biology and Endocrinology (2018) IF: 2.852
    Alleviation of endoplasmic reticulum stress protects against cisplatin-induced ovarian damage

    DOI: 10.1186/s12958-018-0404-4

    PMID: 30176887

    		 Sample: Plasma
  43. Analytical Cellular Pathology (2021) IF: 2.916
    Establishment and Mechanism Study of a Primary Ovarian Insufficiency Mouse Model Using Lipopolysaccharide

    DOI: 10.1155/2021/1781532
    Sample: serum
  44. Chinese Medicine (2020) IF: 2.96
    Catechins from oolong tea improve uterine defects by inhibiting STAT3 signaling in polycystic ovary syndrome mice

    DOI: 10.1186/s13020-020-00405-y

    PMID: 33292347

    		 Sample: serum
  45. REPRODUCTION FERTILITY AND DEVELOPMENT (2020) IF: 1.721
    High-fat diet leads to elevated lipid accumulation and endoplasmic reticulum stress in oocytes, causing poor embryo development

    DOI: 10.1071/RD20112

    PMID: 32998795

    		 Sample: blood
  46. DRUG AND CHEMICAL TOXICOLOGY (2019) IF: 1.946
    Fluted pumpkin seeds protect against busulfan-induced oxidative stress and testicular injuries in adult mice

    DOI: 10.1080/01480545.2019.1657885

    PMID: 31469002

    		 Sample: Plasma
  47. REPRODUCTION FERTILITY AND DEVELOPMENT (2022) IF: 1.973
    Anti-tuberculosis drugs used in a directly observed treatment short course (DOTS) schedule alter endocrine patterns and reduce the ovarian reserve and oocyte quality in the mouse

    DOI: 10.1071/RD22108

    PMID: 36219878

    		 Sample: serum
  48. TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH (2021) IF: 0.533
    Effect of total flavonoids of Cuscuta chinensis Lam. (Convolvulaceae) on oxidative stress injury in mouse testis and epididymis, and on serum levels of reproductive hormones in oligoasthenospermia mice model

    DOI: 10.4314/tjpr.v18i6.15
    Sample: Serum,Tissue homogenate
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