Mouse GAL7 (Galectin 7) CLIA Kit (E-CL-M0322)

Name: Mouse GAL7 (Galectin 7) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0322
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse GAL7 in samples.No significant cross-reactivity or interference between Mouse GAL7 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	57357.000	59515.000	58436.000	58404
1000	24159.000	25651.000	24905.000	24873
500	12254.000	11392.000	11823.000	11791
250	5330.000	6274.000	5802.000	5770
125	2853.000	2729.000	2791.000	2759
62.5	1531.000	1351.000	1441.000	1409
31.25	668.000	748.000	708.000	676
0	31.000	33.000	32.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	91.860	188.530	965.810	89.180	188.970	958.630
Standard deviation	11.790	20.910	99.290	8.670	16.910	62.310
CV（%）	12.830	11.090	10.280	9.720	8.950	6.500

Recovery

The recovery of Mouse GAL7 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	98-112	106
EDTA plasma （n=8）	101-115	107
Cell culture media （n=8）	100-116	108

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse GAL7 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	87-97	95-109	99-112
1:2	Average	92	101	105
1:4	Range	94-105	91-105	103-116
1:4	Average	99	98	109
1:8	Range	100-114	85-96	95-108
1:8	Average	107	91	102

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GAL7. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GAL7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GAL7, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse GAL7. You can calculate the concentration of Mouse GAL7 in the samples by comparing the RLU value of the samples to the standard curve.

Background