Mouse GH (Growth Hormone) CLIA Kit (E-CL-M0054)

Name: Mouse GH (Growth Hormone) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0054
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse GH in samples.No significant cross-reactivity or interference between Mouse GH and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	58191.000	66251.000	62221.000	62179
2000	26432.000	30178.000	28305.000	28263
1000	14956.000	13102.000	14029.000	13987
500	6598.000	7852.000	7225.000	7183
250	3755.000	3313.000	3534.000	3492
125	1774.000	1566.000	1670.000	1628
62.5	786.000	864.000	825.000	783
0	42.000	42.000	42.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	203.340	608.150	1950.460	186.320	551.640	1807.140
Standard deviation	22.900	72.550	163.250	18.840	52.520	159.030
CV（%）	11.260	11.930	8.370	10.110	9.520	8.800

Recovery

The recovery of Mouse GH was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	93-108	101
EDTA plasma （n=8）	86-96	91
Cell culture media （n=8）	98-111	104

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse GH and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	94-108	91-108	85-97
1:2	Average	102	98	91
1:4	Range	99-117	94-105	100-117
1:4	Average	107	99	109
1:8	Range	96-113	87-98	86-98
1:8	Average	104	93	92

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GH. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GH, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse GH. You can calculate the concentration of Mouse GH in the samples by comparing the RLU value of the samples to the standard curve.

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