Mouse GLP-1 (Glucagon Like Peptide 1)CLIA Kit (E-CL-M0077)

Name: Mouse GLP-1 (Glucagon Like Peptide 1)CLIA Kit
Brand: Elabscience
SKU: E-CL-M0077
Price: 180.0 USD
Availability: InStock

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Mouse GLP-1 (Glucagon Like Peptide 1)CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse GLP-1 in samples.No significant cross-reactivity or interference between Mouse GLP-1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	49710.000	60070.000	54890.000	54857
250	22599.000	23189.000	22894.000	22861
125	10138.000	9110.000	9624.000	9591
62.5	4481.000	5265.000	4873.000	4840
31.25	2698.000	2298.000	2498.000	2465
15.63	1395.000	1259.000	1327.000	1294
7.81	724.000	780.000	752.000	719
0	32.000	34.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	22.830	42.110	175.020	25.050	39.520	192.100
Standard deviation	2.790	4.800	13.770	3.100	4.630	14.470
CV（%）	12.220	11.400	7.870	12.380	11.720	7.530

Recovery

The recovery of Mouse GLP-1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	96-110	102
EDTA plasma （n=8）	88-98	93
Cell culture media （n=8）	94-105	100

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse GLP-1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	92-108	101-119	96-109
1:2	Average	99	110	103
1:4	Range	99-114	101-118	96-110
1:4	Average	106	108	104
1:8	Range	86-98	85-99	88-99
1:8	Average	92	91	94

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GLP-1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GLP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GLP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse GLP-1. You can calculate the concentration of Mouse GLP-1 in the samples by comparing the RLU value of the samples to the standard curve.

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