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Mouse GM-CSF(Granulocyte-Macrophage Colony Stimulating Factor) ELISA Kit

  • Cat.No.:E-EL-M0032

  • Reactivity: Mouse

To Purchase E-EL-M0032

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse GM-CSF in samples. No significant cross-reactivity or interference between Mouse GM-CSF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse GM-CSF concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GM-CSF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GM-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GM-CSF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse GM-CSF. You can calculate the concentration of Mouse GM-CSF in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse GM-CSF were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse GM-CSF were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 47.77 95.17 346.82 46.75 93.81 328.61
Standard deviation 2.64 5.61 17.41 3.02 4.58 17.88
CV (%) 5.53 5.89 5.02 6.46 4.88 5.44


The recovery of Mouse GM-CSF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-106 101
EDTA plasma (n=8) 85-99 91
Cell culture media (n=8) 93-108 101

Target Information

Database LinksSwissProt:   P01587
SynonymsGMCSF, CSF2
Research AreaCancer, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


  1. Bioactive Materials (2021) IF: 14.593
    Secretions from hypochlorous acid-treated tumor cells delivered in a melittin hydrogel potentiate cancer immunotherapy

    DOI: 10.1016/j.bioactmat.2021.07.019

    PMID: 34820587

    Sample: B16–F10 cells,Red blood cells
  2. Frontiers in Immunology (2022) IF: 8.786
    Gut Microflora Modulates Th17/Treg Cell Differentiation in Experimental Autoimmune Prostatitis via the Short-Chain Fatty Acid Propionate

    DOI: 10.3389/fimmu.2022.915218

    PMID: 35860242

    Sample: serum
  3. Journal of Ginseng Research (2020) IF: 5.487
    Inhibitory activity of gintonin on inflammation in human IL-1β-stimulated fibroblast-like synoviocytes and collagen-induced arthritis in mice

    DOI: 10.1016/j.jgr.2020.12.001

    PMID: 34295211

    Sample: serum
  4. Frontiers in Pharmacology (2022) IF: 5.811
    The Effect of Shionone on Sepsis-Induced Acute Lung Injury by the ECM1/STAT5/NF-κB Pathway

    DOI: 10.3389/fphar.2021.764247

    Sample: serum,RAW264.7 cell
    Danggui Buxue Decoction enhances the anticancer activity of gemcitabine and alleviates gemcitabine-induced myelosuppression

    DOI: 10.1016/j.jep.2021.113965

    PMID: 33639205

    Sample: Serum,Tissue(spleen)
  6. FASEB JOURNAL (2020) IF: 4.966
    CaMK4‐dependent phosphorylation of Akt/mTOR underlies Th17 excessive activation in experimental autoimmune prostatitis

    DOI: 10.1096/fj.201902910RRR

    Sample: Cell culture supernatant,Tissue homogenate,serum
  7. CHEMISTRY & BIODIVERSITY (2021) IF: 2.408
    Shengxuening Extracted from Silkworm Excrement Mitigates Myelosuppression via SCF-Mediated JAK2/STAT3 Signaling

    DOI: 10.1002/cbdv.202100139

    PMID: 33973702

    Sample: Serum
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