Mouse HC Ⅱ(Heparin Cofactor Ⅱ) CLIA Kit (E-CL-M0405)

Name: Mouse HC Ⅱ(Heparin Cofactor Ⅱ) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0405
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Mouse HC Ⅱ(Heparin Cofactor Ⅱ) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse HC Ⅱ in samples.No significant cross-reactivity or interference between Mouse HC Ⅱ and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	400321.000	408754.000	404537.500	402959
1000	354467.000	356301.000	355384.000	353805.5
500	279454.000	281347.000	280400.500	278822
250	227418.000	224980.000	226199.000	224620.5
125	158941.000	149578.000	154259.500	152681
62.5	79987.000	79843.000	79915.000	78336.5
31.25	36791.000	35145.000	35968.000	34389.5
0	1632.000	1525.000	1578.500	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	92.970	293.330	760.160	100.830	307.970	729.610
Standard deviation	5.980	16.020	26.070	5.890	17.520	33.490
CV（%）	6.430	5.460	3.430	5.840	5.690	4.590

Recovery

The recovery of Mouse HCⅡ was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-105	96
EDTA plasma （n=8）	88-103	94
Cell culture media （n=8）	90-102	96

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse HCⅡ and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	100-111	91-105	88-103
1:2	Average	106	97	95
1:4	Range	101-117	90-102	86-99
1:4	Average	107	96	93
1:8	Range	100-114	93-108	89-99
1:8	Average	107	99	94

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HC Ⅱ. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HC Ⅱ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HC Ⅱ, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse HC Ⅱ. You can calculate the concentration of Mouse HC Ⅱ in the samples by comparing the RLU value of the samples to the standard curve.