Mouse Hepc (Hepcidin) CLIA Kit (E-CL-M0415)

Name: Mouse Hepc (Hepcidin) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0415
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse Hepc in samples.No significant cross-reactivity or interference between Mouse Hepc and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	58038.000	63512.000	60775.000	60745
1000	23170.000	25456.000	24313.000	24283
500	12490.000	11090.000	11790.000	11760
250	5413.000	5551.000	5482.000	5452
125	3055.000	2657.000	2856.000	2826
62.5	1452.000	1324.000	1388.000	1358
31.25	685.000	725.000	705.000	675
0	30.000	30.000	30.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	110.040	302.390	988.500	100.070	298.430	904.190
Standard deviation	10.270	26.190	79.480	10.590	34.320	81.200
CV（%）	9.330	8.660	8.040	10.580	11.500	8.980

Recovery

The recovery of Mouse Hepc was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-101	95
EDTA plasma （n=8）	86-99	92
Cell culture media （n=8）	83-96	90

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse Hepc and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	97-114	92-108	90-102
1:2	Average	104	99	96
1:4	Range	88-100	97-112	103-117
1:4	Average	95	104	109
1:8	Range	90-102	89-100	90-104
1:8	Average	96	94	96

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse Hepc. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Hepc and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Hepc, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse Hepc. You can calculate the concentration of Mouse Hepc in the samples by comparing the RLU value of the samples to the standard curve.

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