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Mouse Hepc(Hepcidin) ELISA Kit

  • Cat.No.:E-EL-M0671

  • Reactivity: Mouse

To Purchase E-EL-M0671

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 125.00-8000 pg/mL
Sensitivity 75.00 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse Hepc in samples. No significant cross-reactivity or interference between Mouse Hepc and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse Hepc concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Hepc. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Hepc and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Hepc, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Hepc. You can calculate the concentration of Mouse Hepc in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse Hepc were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse Hepc were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 385.00 686.40 3381.60 352.70 696.90 3600.60
Standard deviation 23.50 30.20 121.70 24.70 38.30 144.00
CV (%) 6.10 4.40 3.60 7.00 5.50 4.00

Recovery

The recovery of Mouse Hepc spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-101 94
EDTA plasma (n=8) 89-103 97
Cell culture media (n=8) 89-102 94

Linearity

Samples were spiked with high concentrations of Mouse Hepc and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: Q9EQ21
SynonymsHepcidin Antimicrobial Peptide

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Antioxidants (2020) IF: 5.014
    Epigallocatechin-3-Gallate (EGCG)-Inducible SMILE Inhibits STAT3-Mediated Hepcidin Gene Expression

    DOI: 10.3390/antiox9060514

    PMID: 32545266

    Sample: Serum,Cell culture medium
  2. Frontiers in immunology (2019) IF: 5.511
    Ferritin Light Chain Confers Protection Against Sepsis-Induced Inflammation and Organ Injury

    DOI: 10.3389/fimmu.2019.00131

    PMID: 30804939

    Sample: Serum
  3. Nutrients (2020) IF: 4.546
    Omega-3 Fatty Acid and Iron Supplementation Alone, but Not in Combination, Lower Inflammation and Anemia of Infection in Mycobacterium tuberculosis-Infected Mice

    DOI: 10.3390/nu12092897

    PMID: 32971969

    Sample: Plasma
  4. Journal of molecular endocrinology (2018) IF: 3.577
    Hepcidin is an endogenous protective factor for osteoporosis by reducing iron levels.

    DOI: 10.1530/JME-17-0301

    PMID: 29563156

  5. International Journal of Clinical and Experimental Pathology (2015) IF: 1.706
    An Unusual Case Of Iron Deficiency Anemia Is Associated With Extremely Low Level Of Transferrin Receptor.

    PMID: 26339443

    Sample: Serum
  6. Acta Veterinaria Hungarica (2019) IF: 0.991
    Investigation of sphingosin-1-phosphate-triggered matriptase activation using a rat primary hepatocyte model

    DOI: 10.1556/004.2019.057

    PMID: 31842605

    Sample: Cell culture supernatant
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