Mouse hs-CRP (high sensitivity C-Reactive Protein) CLIA Kit (E-CL-M0420)

Name: Mouse hs-CRP (high sensitivity C-Reactive Protein) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0420
Price: 180.0 USD
Availability: InStock

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Mouse hs-CRP (high sensitivity C-Reactive Protein) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse hs-CRP in samples.No significant cross-reactivity or interference between Mouse hs-CRP and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	55074.000	57500.000	56287.000	56261
500	23317.000	25015.000	24166.000	24140
250	11421.000	10703.000	11062.000	11036
125	4896.000	5016.000	4956.000	4930
62.5	2746.000	2604.000	2675.000	2649
31.25	1375.000	1191.000	1283.000	1257
15.63	738.000	756.000	747.000	721
0	25.000	27.000	26.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	50.120	139.380	366.280	47.140	132.970	400.030
Standard deviation	4.420	11.050	24.140	5.570	10.480	27.320
CV（%）	8.820	7.930	6.590	11.820	7.880	6.830

Recovery

The recovery of Mouse hs-CRP was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	100-112	106
EDTA plasma （n=8）	86-101	92
Cell culture media （n=8）	100-116	108

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse hs-CRP and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	89-102	92-106	83-99
1:2	Average	96	99	90
1:4	Range	87-100	101-115	101-116
1:4	Average	93	107	109
1:8	Range	89-100	97-113	93-107
1:8	Average	94	104	98

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse hs-CRP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse hs-CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse hs-CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse hs-CRP. You can calculate the concentration of Mouse hs-CRP in the samples by comparing the RLU value of the samples to the standard curve.

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