Mouse IL-4 (Interleukin 4) CLIA Kit (E-CL-M0041)

Name: Mouse IL-4 (Interleukin 4) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0041
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	3.75 pg/mL
Detection Range	6.25-400 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse IL-4 in samples.No significant cross-reactivity or interference between Mouse IL-4 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
400	45091.000	51859.000	48475.000	48433
200	19177.000	20705.000	19941.000	19899
100	9449.000	8899.000	9174.000	9132
50	4046.000	4298.000	4172.000	4130
25	2327.000	2111.000	2219.000	2177
12.5	1236.000	1076.000	1156.000	1114
6.25	711.000	765.000	738.000	696
0	42.000	42.000	42.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	21.440	57.260	142.710	22.340	59.530	129.610
Standard deviation	2.570	4.570	12.470	2.480	5.640	13.740
CV（%）	11.990	7.980	8.740	11.100	9.470	10.600

Recovery

The recovery of Mouse IL-4 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	99-114	105
EDTA plasma （n=8）	86-101	93
Cell culture media （n=8）	97-114	104

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse IL-4 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	86-102	97-110	95-109
1:2	Average	93	104	103
1:4	Range	98-114	91-101	97-112
1:4	Average	107	96	105
1:8	Range	84-96	91-104	85-97
1:8	Average	91	98	91

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-4. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-4, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse IL-4. You can calculate the concentration of Mouse IL-4 in the samples by comparing the RLU value of the samples to the standard curve.

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