Mouse IL-6 (Interleukin 6) CLIA Kit (E-CL-M0042)

Name: Mouse IL-6 (Interleukin 6) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0042
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse IL-6 in samples.No significant cross-reactivity or interference between Mouse IL-6 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	52107.000	61191.000	56649.000	56607
250	21995.000	23893.000	22944.000	22902
125	9700.000	9498.000	9599.000	9557
62.5	4241.000	4831.000	4536.000	4494
31.25	2470.000	2242.000	2356.000	2314
15.63	1346.000	1322.000	1334.000	1292
7.81	745.000	829.000	787.000	745
0	41.000	43.000	42.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	25.380	43.040	236.410	25.670	46.390	259.780
Standard deviation	2.230	4.740	17.050	3.110	4.630	29.590
CV（%）	8.790	11.010	7.210	12.120	9.980	11.390

Recovery

The recovery of Mouse IL-6 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	86-99	92
EDTA plasma （n=8）	92-107	98
Cell culture media （n=8）	88-101	95

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse IL-6 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	100-118	104-116	96-110
1:2	Average	107	110	103
1:4	Range	91-105	89-100	91-106
1:4	Average	97	94	97
1:8	Range	93-105	94-108	86-98
1:8	Average	98	101	91

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-6. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse IL-6. You can calculate the concentration of Mouse IL-6 in the samples by comparing the RLU value of the samples to the standard curve.

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