Mouse LEP (Leptin) CLIA Kit (E-CL-M0037)

Name: Mouse LEP (Leptin) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0037
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse LEP in samples.No significant cross-reactivity or interference between Mouse LEP and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	53492.000	63830.000	58661.000	58626
500	21303.000	25549.000	23426.000	23391
250	11167.000	9687.000	10427.000	10392
125	4993.000	5515.000	5254.000	5219
62.5	2714.000	2634.000	2674.000	2639
31.25	1303.000	1287.000	1295.000	1260
15.63	692.000	724.000	708.000	673
0	33.000	37.000	35.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	47.040	113.360	422.100	45.660	118.870	414.180
Standard deviation	5.830	12.570	30.690	5.720	9.130	34.210
CV（%）	12.390	11.090	7.270	12.530	7.680	8.260

Recovery

The recovery of Mouse LEP was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-106	97
EDTA plasma （n=8）	85-97	91
Cell culture media （n=8）	88-102	93

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse LEP and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	99-115	91-106	103-118
1:2	Average	107	98	109
1:4	Range	85-97	88-103	101-115
1:4	Average	90	95	107
1:8	Range	90-103	87-101	100-115
1:8	Average	97	93	108

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse LEP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LEP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse LEP. You can calculate the concentration of Mouse LEP in the samples by comparing the RLU value of the samples to the standard curve.

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