Mouse LZM (Lysozyme) CLIA Kit (E-CL-M0486)

Name: Mouse LZM (Lysozyme) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0486
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse LZM in samples.No significant cross-reactivity or interference between Mouse LZM and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	56881.000	62543.000	59712.000	59679
1000	24146.000	24224.000	24185.000	24152
500	11764.000	10750.000	11257.000	11224
250	5156.000	5588.000	5372.000	5339
125	2781.000	2529.000	2655.000	2622
62.5	1502.000	1312.000	1407.000	1374
31.25	675.000	767.000	721.000	688
0	33.000	33.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	108.570	272.510	982.440	98.350	249.660	934.720
Standard deviation	11.770	23.680	98.830	11.970	22.440	92.260
CV（%）	10.840	8.690	10.060	12.170	8.990	9.870

Recovery

The recovery of Mouse LZM was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-102	94
EDTA plasma （n=8）	95-110	102
Cell culture media （n=8）	86-97	92

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse LZM and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	94-109	100-113	99-112
1:2	Average	100	107	105
1:4	Range	99-116	97-112	96-108
1:4	Average	106	104	102
1:8	Range	86-99	99-111	94-106
1:8	Average	92	104	99

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse LZM. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LZM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LZM, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse LZM. You can calculate the concentration of Mouse LZM in the samples by comparing the RLU value of the samples to the standard curve.

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