Mouse MAU(Microalbuminuria) ELISA Kit

  • Cat.No.:E-EL-M0792

  • Reactivity: Mouse

To Purchase E-EL-M0792

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse MAU in samples. No significant cross-reactivity or interference between Mouse MAU and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse MAU concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MAU. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MAU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MAU, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MAU. You can calculate the concentration of Mouse MAU in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse MAU were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse MAU were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.45 1.17 4.29 0.48 1.13 4.64
Standard deviation 0.02 0.06 0.22 0.03 0.05 0.22
CV (%) 4.44 5.13 5.13 6.25 4.42 4.74


The recovery of Mouse MAU spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-101 96
EDTA plasma (n=8) 86-96 90
Cell culture media (n=8) 100-107 104


Samples were spiked with high concentrations of Mouse MAU and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsUMA, mAlb

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Biomedicine & Pharmacotherapy (2019) IF: 4.545
    Therapeutic potential of NaoXinTong Capsule on the developed diabetic nephropathy in db/db mice

    DOI: 10.1016/j.biopha.2019.109389

    PMID: 31545275

    Sample: Serum,Urine
  2. Scientific reports (2018) IF: 4.122
    NaoXinTong Capsules inhibit the development of diabetic nephropathy in db/db mice.

    DOI: 10.1038/s41598-018-26746-1

    PMID: 29904053

    Sample: Urine
  3. Biochemical & Biophysical Research Communications (2016) IF: 2.466
    Overexpression Of Foxo1 Ameliorates The Podocyte Epithelial-Mesenchymal Transition Induced By High Glucose InVitro And InVivo.

    DOI: 10.1016/j.bbrc.2016.02.066

    PMID: 26902117

    Sample: Urine
  4. Acta Biochimica Et Biophysica Sinica (2016) IF: 2.2
    Mup-Knockout Mice Generated Through CRISPR/Cas9-Mediated Deletion For Use In Urinary Protein Analysis.

    DOI: 10.1093/abbs/gmy111

    PMID: 30215664

    Sample: Urine
  5. Theranostics (2021) IF: 8.5790
    GPR43 deficiency protects against podocyte insulin resistance in diabetic nephropathy through the restoration of AMPKα activity

    DOI: 10.7150

    PMID: 99999999

    Sample: Urine
  6. Theranostics (2021) IF: 8.5790
    Mechanism of the diuretic activity of Descurainia sophia seed.

    DOI: 10.7150

    PMID: 99999999

    Sample: Urine
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