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Mouse MAU(Microalbuminuria) ELISA Kit

Cat.No.:E-EL-M0792
Reactivity: Mouse

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96T
48T
24T
96T*5
96T*10

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse MAU in samples. No significant cross-reactivity or interference between Mouse MAU and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse MAU concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MAU. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MAU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MAU, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MAU. You can calculate the concentration of Mouse MAU in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse MAU were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse MAU were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.45	1.17	4.29	0.48	1.13	4.64
Standard deviation	0.02	0.06	0.22	0.03	0.05	0.22
CV (%)	4.44	5.13	5.13	6.25	4.42	4.74

Recovery

The recovery of Mouse MAU spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	89-101	96
EDTA plasma (n=8)	86-96	90
Cell culture media (n=8)	100-107	104

Linearity

Samples were spiked with high concentrations of Mouse MAU and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	UMA
Research Area	Cancer, Cardiovascular, Metabolism, Developmental biology, Signal transduction, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Nature Communications (2023) IF: 17.694
Long-term statins administration exacerbates diabetic nephropathy via ectopic fat deposition in diabetic mice

DOI: 10.1038/s41467-023-35944-z

PMID: 36693830
Sample: urine
DIABETES (2023) IF: 9.337
Metrnl alleviates lipid accumulation by modulating mitochondrial homeostasis in diabetic nephropathy

DOI: 10.2337/db22-0680

Sample: renal cortex,serum
Cell Death & Disease (2022) IF: 8.4689
BMP-7 ameliorates partial epithelial-mesenchymal transition by restoring SnoN protein level via Smad1/5 pathway in diabetic kidney disease

DOI: 10.1038/s41419-022-04529-x

PMID: 35314669
Sample: Urine
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
Bisdemethoxycurcumin Attenuated Renal Injury via Activation of Keap1/Nrf2 Pathway in High-Fat Diet-Fed Mice

DOI: 10.3390/ijms23137395

PMID: 35806399
Sample: urinary
Microbial Biotechnology (2022) IF: 6.575
Abelmoschus Manihot ameliorates the levels of circulating metabolites in diabetic nephropathy by modulating gut microbiota in non-obese diabetes mice

DOI: 10.1111/1751-7915.14200

Sample: urine
Metabolites (2022) IF: 5.581
Interaction between Plasma Metabolomics and Intestinal Microbiome in db/db Mouse, an Animal Model for Study of Type 2 Diabetes and Diabetic Kidney Disease

DOI: 10.3390/metabo12090775

PMID: 36144180
Sample: urine
FASEB JOURNAL (2023) IF: 5.834
miR-124-3p improves mitochondrial function of renal tubular epithelial cells in db/db mice

DOI: 10.1096/fj.202201202RR

Sample: urine
Journal of Cell Communication and Signaling (2022) IF: 5.908
Prediction of cellular targets in diabetic kidney diseases with single-cell transcriptomic analysis of db/db mouse kidneys

DOI: 10.1007/s12079-022-00685-z

PMID: 35809207
Sample: urine
Scientific Reports (2018) IF: 4.122
NaoXinTong Capsules inhibit the development of diabetic nephropathy in db/db mice

DOI: 10.1038/s41598-018-26746-1

Sample: Urine
ARTHRITIS RESEARCH & THERAPY (2019) IF: 4.148
ALW peptide ameliorates lupus nephritis in MRL/lpr mice

DOI: 10.1186/s13075-019-2038-0

Sample: urine
Drug Design Development and Therapy (2021) IF: 4.162
Network Pharmacology Analysis of ZiShenWan for Diabetic Nephropathy and Experimental Verification of Its Anti-Inflammatory Mechanism

DOI: 10.2147/DDDT.S297683

PMID: 33883881
Sample: Whole Blood,Urine,Tissue homogenate
BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
Marein ameliorates diabetic nephropathy by inhibiting renal sodium glucose transporter 2 and activating the AMPK signaling pathway in db/db mice and high glucose–treated HK-2 cells

DOI: 10.1016/j.biopha.2020.110684

Sample: kidney
TOXICOLOGY LETTERS (2020) IF: 3.569
Local renal complement activation mediates immune kidney injury by inducing endothelin-1 signalling and inflammation in trichloroethylene-sensitised mice

DOI: 10.1016/j.toxlet.2020.07.036

Sample: urine
BIOMEDICINE & PHARMACOTHERAPY (2019) IF: 3.743
Therapeutic potential of NaoXinTong Capsule on the developed diabetic nephropathy in db/db mice

DOI: 10.1016/j.biopha.2019.109389

PMID: 31545275
Sample: Serum,Urine
ACTA BIOCHIMICA ET BIOPHYSICA SINICA (2016) IF: 2.124
Mup-knockout mice generated through CRISPR/Cas9-mediated deletion for use in urinary protein analysis

DOI: 10.1093/abbs/gmw003

Sample: urine
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2016) IF: 2.371
Overexpression of FOXO1 ameliorates the podocyte epithelial–mesenchymal transition induced by high glucose in vitro and in vivo

DOI: 10.1016/j.bbrc.2016.02.066

Sample: Urine

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