Mouse MBP (Myelin Basic Protein) CLIA Kit (E-CL-M0504)

Name: Mouse MBP (Myelin Basic Protein) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0504
Price: 180.0 USD
Availability: InStock

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Mouse MBP (Myelin Basic Protein) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse MBP in samples.No significant cross-reactivity or interference between Mouse MBP and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	58733.000	59785.000	59259.000	59226
1000	23233.000	25033.000	24133.000	24100
500	12236.000	12014.000	12125.000	12092
250	5157.000	5951.000	5554.000	5521
125	2942.000	2586.000	2764.000	2731
62.5	1394.000	1386.000	1390.000	1357
31.25	659.000	697.000	678.000	645
0	33.000	33.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	108.580	279.340	966.850	105.030	254.610	894.570
Standard deviation	12.700	24.890	64.680	11.100	19.100	63.510
CV（%）	11.700	8.910	6.690	10.570	7.500	7.100

Recovery

The recovery of Mouse MBP was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	100-113	106
EDTA plasma （n=8）	92-105	99
Cell culture media （n=8）	91-107	97

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse MBP and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	86-99	101-114	101-119
1:2	Average	92	108	109
1:4	Range	86-100	89-100	95-110
1:4	Average	91	95	101
1:8	Range	99-110	86-101	90-105
1:8	Average	104	92	96

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MBP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MBP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse MBP. You can calculate the concentration of Mouse MBP in the samples by comparing the RLU value of the samples to the standard curve.

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