Mouse MMP-9 (Matrix Metalloproteinase-9) CLIA Kit (E-CL-M0381)

Name: Mouse MMP-9 (Matrix Metalloproteinase-9) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0381
Price: 180.0 USD
Availability: InStock

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Mouse MMP-9 (Matrix Metalloproteinase-9) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse MMP-9 in samples.No significant cross-reactivity or interference between Mouse MMP-9 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	56828.000	60158.000	58493.000	58458
1000	24418.000	24524.000	24471.000	24436
500	12842.000	11332.000	12087.000	12052
250	5107.000	5723.000	5415.000	5380
125	2724.000	2696.000	2710.000	2675
62.5	1540.000	1334.000	1437.000	1402
31.25	690.000	788.000	739.000	704
0	33.000	37.000	35.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	97.020	230.320	864.990	100.980	241.510	929.460
Standard deviation	11.250	25.980	54.840	10.310	21.230	94.150
CV（%）	11.600	11.280	6.340	10.210	8.790	10.130

Recovery

The recovery of Mouse MMP-9 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	94-110	100
EDTA plasma （n=8）	94-109	101
Cell culture media （n=8）	101-117	107

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse MMP-9 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	90-105	93-107	98-115
1:2	Average	97	100	106
1:4	Range	98-115	90-101	88-101
1:4	Average	106	96	95
1:8	Range	93-105	87-101	87-101
1:8	Average	100	94	93

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse MMP-9. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MMP-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MMP-9, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse MMP-9. You can calculate the concentration of Mouse MMP-9 in the samples by comparing the RLU value of the samples to the standard curve.

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