Mouse NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) CLIA Kit (E-CL-M0431)

Name: Mouse NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0431
Price: 180.0 USD
Availability: InStock

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Mouse NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse NOS2/iNOS in samples.No significant cross-reactivity or interference between Mouse NOS2/iNOS and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	62177.000	66241.000	64209.000	64177
2000	27576.000	29752.000	28664.000	28632
1000	15134.000	14088.000	14611.000	14579
500	6725.000	7067.000	6896.000	6864
250	3346.000	3282.000	3314.000	3282
125	1724.000	1480.000	1602.000	1570
62.5	795.000	857.000	826.000	794
0	32.000	32.000	32.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	213.230	560.810	1523.990	192.810	556.720	1413.080
Standard deviation	19.300	40.880	134.110	21.400	48.820	153.460
CV（%）	9.050	7.290	8.800	11.100	8.770	10.860

Recovery

The recovery of Mouse NOS2 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	88-102	95
EDTA plasma （n=8）	89-102	96
Cell culture media （n=8）	90-104	96

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse NOS2 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	88-101	103-118	90-103
1:2	Average	94	110	97
1:4	Range	101-117	93-104	99-116
1:4	Average	109	99	107
1:8	Range	98-115	89-102	97-110
1:8	Average	105	96	104

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse NOS2/iNOS. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NOS2/iNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NOS2/iNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse NOS2/iNOS. You can calculate the concentration of Mouse NOS2/iNOS in the samples by comparing the RLU value of the samples to the standard curve.

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