Mouse NRG-1 (Neuregulin 1) CLIA Kit (E-CL-M0087)

Name: Mouse NRG-1 (Neuregulin 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0087
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse NRG-1 in samples.No significant cross-reactivity or interference between Mouse NRG-1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	54122.000	62850.000	58486.000	58450
500	22123.000	27029.000	24576.000	24540
250	11268.000	10236.000	10752.000	10716
125	4896.000	5460.000	5178.000	5142
62.5	2641.000	2417.000	2529.000	2493
31.25	1451.000	1283.000	1367.000	1331
15.63	721.000	821.000	771.000	735
0	35.000	37.000	36.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	53.090	152.320	374.880	57.920	144.190	370.580
Standard deviation	6.820	11.030	36.700	6.240	12.430	27.900
CV（%）	12.850	7.240	9.790	10.770	8.620	7.530

Recovery

The recovery of Mouse NRG-1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	93-107	100
EDTA plasma （n=8）	87-99	92
Cell culture media （n=8）	93-107	101

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse NRG-1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	96-111	96-112	93-110
1:2	Average	104	104	101
1:4	Range	103-116	93-109	84-98
1:4	Average	110	101	91
1:8	Range	83-98	99-115	101-117
1:8	Average	90	107	108

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse NRG-1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NRG-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NRG-1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse NRG-1. You can calculate the concentration of Mouse NRG-1 in the samples by comparing the RLU value of the samples to the standard curve.

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