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Mouse NT-proBNP(N-terminal pro-Brain Natriuretic Peptide) ELISA Kit

Citations(16)

Cat.No.:E-EL-M0834
Reactivity: Mouse

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96T
48T
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96T*5
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse NT-proBNP in samples. No significant cross-reactivity or interference between Mouse NT-proBNP and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse NT-proBNP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NT-proBNP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NT-proBNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NT-proBNP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse NT-proBNP. You can calculate the concentration of Mouse NT-proBNP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 12 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 12 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 12 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse NT-proBNP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse NT-proBNP were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	45.76	89.12	350.68	42.25	85.48	364.48
Standard deviation	2.86	5.07	11.12	2.11	3.44	18.26
CV (%)	6.25	5.69	3.17	4.99	4.02	5.01

Recovery

The recovery of Mouse NT-proBNP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-100	91
EDTA plasma (n=8)	95-108	101
Cell culture media (n=8)	93-103	98

Linearity

Samples were spiked with high concentrations of Mouse NT-proBNP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	NT-Pro-BNP
Research Area	Cancer, Cardiovascular, Neuroscience

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Acta Pharmaceutica Sinica B (2022) IF: 14.903

Compound Danshen Dripping Pill inhibits hypercholesterolemia/atherosclerosis-induced heart failure in ApoE and LDLR dual deficient mice via multiple mechanisms

DOI: 10.1016/j.apsb.2022.11.012
	Sample: Heart

Frontiers in Cardiovascular Medicine (2022) IF: 6.05

Momordica. charantia-Derived Extracellular Vesicles-Like Nanovesicles Protect Cardiomyocytes Against Radiation Injury via Attenuating DNA Damage and Mitochondria Dysfunction

DOI: 10.3389/fcvm.2022.864188
PMID: 35509278	Sample: Serum

Natural Product Communications (2022) IF: 1.496

Phillyrin Inhibits Isoproterenol-Induced Cardiac Hypertrophy Via P38 and NF-κB Pathways

DOI: 10.1177/1934578X221144581
	Sample: Serum

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2018) IF: 2.784

Opening of mitoKATP improves cardiac function and inhibits apoptosis via the AKT-Foxo1 signaling pathway in diabetic cardiomyopathy

DOI: 10.3892/ijmm.2018.3832
PMID: 30132505	Sample: Serum,Cell Culture Supernatant

Parasites & Vectors (2020) IF: 2.824

Therapeutic efficacy of Schistosoma japonicum cystatin on sepsis-induced cardiomyopathy in a mouse model

DOI: 10.1186/s13071-020-04104-3
PMID: 32423469	Sample: Serum

Molecular Medicine Reports (2021) IF: 2.952

Role of Thrombospondin‑1 in sepsis‑induced myocardial injury

DOI: 10.3892/mmr.2021.12509
	Sample: Myocardial Primary Cell

Cardiovascular Therapeutics (2022) IF: 3.023

L-Carnitine Alleviates the Myocardial Infarction and Left Ventricular Remodeling through Bax/Bcl-2 Signal Pathway

DOI: 10.1155/2022/9615674
PMID: 35692375	Sample: Plasma

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2017) IF: 3.226

Elevation of Serum APE1/Ref-1 in Experimental Murine Myocarditis

DOI: 10.3390/ijms18122664
	Sample: Serum

Scientific Reports (2020) IF: 3.998

Administration of apo A-I (Milano) nanoparticles reverses pathological remodelling, cardiac dysfunction, and heart failure in a murine model of HFpEF associated with hypertension

DOI: 10.1038/s41598-020-65255-y
PMID: 32433476	Sample: Plasma

American Journal of Translational Research (2020) IF: 4.06

The mobilization of splenic reservoir myeloid-derived suppressor cells in sepsis-induced myocardial injury


PMID: 33312354	Sample: Serum

Neuroscience Bulletin (2020) IF: 4.326

Macrophage–NLRP3 Inflammasome Activation Exacerbates Cardiac Dysfunction after Ischemic Stroke in a Mouse Model of Diabetes

DOI: 10.1007/s12264-020-00544-0
	Sample: Plasma

EUROPEAN JOURNAL OF PHARMACOLOGY (2022) IF: 4.432

Phillyrin attenuates norepinephrine-induced cardiac hypertrophy and inflammatory response by suppressing p38/ERK1/2 MAPK and AKT/NF-kappaB pathways

DOI: 10.1016/j.ejphar.2022.175022
PMID: 35569549	Sample: Serum

FASEB JOURNAL (2021) IF: 5.192

Apela inhibits systemic and renal inflammatory reactions in mice with type I cardiorenal syndrome

DOI: 10.1096/fj.202101030R
PMID: 34516679	Sample: Plasma

BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53

Compound Danshen Dripping Pill inhibits doxorubicin or isoproterenol-induced cardiotoxicity

DOI: 10.1016/j.biopha.2021.111531
PMID: 34311530	Sample: Serum

BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53

Cardioprotection effect of Yiqi–Huoxue–Jiangzhuo formula in a chronic kidney disease mouse model associated with gut microbiota modulation and NLRP3 inflammasome inhibition

DOI: 10.1016/j.biopha.2022.113159

BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 6.53

ATF4 protects against sorafenib-induced cardiotoxicity by suppressing ferroptosis

DOI: 10.1016/j.biopha.2022.113280
PMID: 35724508	Sample: Serum

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