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Mouse NT-proBNP(N-terminal pro-Brain Natriuretic Peptide) ELISA Kit

  • Cat.No.:E-EL-M0834

  • Reactivity: Mouse

To Purchase E-EL-M0834

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse NT-proBNP in samples. No significant cross-reactivity or interference between Mouse NT-proBNP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse NT-proBNP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NT-proBNP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NT-proBNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NT-proBNP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse NT-proBNP. You can calculate the concentration of Mouse NT-proBNP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse NT-proBNP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse NT-proBNP were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 45.76 89.12 350.68 42.25 85.48 364.48
Standard deviation 2.86 5.07 11.12 2.11 3.44 18.26
CV (%) 6.25 5.69 3.17 4.99 4.02 5.01


The recovery of Mouse NT-proBNP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-100 91
EDTA plasma (n=8) 95-108 101
Cell culture media (n=8) 93-103 98


Samples were spiked with high concentrations of Mouse NT-proBNP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research AreaCancer, Cardiovascular, Neuroscience

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


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View more details about our Publication Reward >>

  1. Acta Pharmaceutica Sinica B (2022) IF: 14.903
    Compound Danshen Dripping Pill inhibits hypercholesterolemia/atherosclerosis-induced heart failure in ApoE and LDLR dual deficient mice via multiple mechanisms

    DOI: 10.1016/j.apsb.2022.11.012

    Sample: heart
  2. Frontiers in Cardiovascular Medicine (2022) IF: 6.05
    Momordica. charantia-Derived Extracellular Vesicles-Like Nanovesicles Protect Cardiomyocytes Against Radiation Injury via Attenuating DNA Damage and Mitochondria Dysfunction

    DOI: 10.3389/fcvm.2022.864188

    PMID: 35509278

    Sample: serum
    Compound Danshen Dripping Pill inhibits doxorubicin or isoproterenol-induced cardiotoxicity

    DOI: 10.1016/j.biopha.2021.111531

    PMID: 34311530

    Sample: Serum
    Cardioprotection effect of Yiqi–Huoxue–Jiangzhuo formula in a chronic kidney disease mouse model associated with gut microbiota modulation and NLRP3 inflammasome inhibition

    DOI: 10.1016/j.biopha.2022.113159

    ATF4 protects against sorafenib-induced cardiotoxicity by suppressing ferroptosis

    DOI: 10.1016/j.biopha.2022.113280

    PMID: 35724508

    Sample: serum
  6. FASEB JOURNAL (2021) IF: 5.192
    Apela inhibits systemic and renal inflammatory reactions in mice with type I cardiorenal syndrome

    DOI: 10.1096/fj.202101030R

    PMID: 34516679

    Sample: plasma
  7. American Journal of Translational Research (2020) IF: 4.06
    The mobilization of splenic reservoir myeloid-derived suppressor cells in sepsis-induced myocardial injury

    PMID: 33312354

    Sample: Serum
  8. Neuroscience Bulletin (2020) IF: 4.326
    Macrophage–NLRP3 Inflammasome Activation Exacerbates Cardiac Dysfunction after Ischemic Stroke in a Mouse Model of Diabetes

    DOI: 10.1007/s12264-020-00544-0

    Sample: Plasma
    Phillyrin attenuates norepinephrine-induced cardiac hypertrophy and inflammatory response by suppressing p38/ERK1/2 MAPK and AKT/NF-kappaB pathways

    DOI: 10.1016/j.ejphar.2022.175022

    PMID: 35569549

    Sample: serum
  10. Cardiovascular Therapeutics (2022) IF: 3.023
    L-Carnitine Alleviates the Myocardial Infarction and Left Ventricular Remodeling through Bax/Bcl-2 Signal Pathway

    DOI: 10.1155/2022/9615674

    PMID: 35692375

    Sample: plasma
    Elevation of Serum APE1/Ref-1 in Experimental Murine Myocarditis

    DOI: 10.3390/ijms18122664

    Sample: Serum
  12. Scientific Reports (2020) IF: 3.998
    Administration of apo A-I (Milano) nanoparticles reverses pathological remodelling, cardiac dysfunction, and heart failure in a murine model of HFpEF associated with hypertension

    DOI: 10.1038/s41598-020-65255-y

    PMID: 32433476

    Sample: Plasma
    Opening of mitoKATP improves cardiac function and inhibits apoptosis via the AKT-Foxo1 signaling pathway in diabetic cardiomyopathy

    DOI: 10.3892/ijmm.2018.3832

    PMID: 30132505

    Sample: Serum,Cell culture supernatant
  14. Parasites & Vectors (2020) IF: 2.824
    Therapeutic efficacy of Schistosoma japonicum cystatin on sepsis-induced cardiomyopathy in a mouse model

    DOI: 10.1186/s13071-020-04104-3

    PMID: 32423469

    Sample: Serum
  15. Molecular Medicine Reports (2021) IF: 2.952
    Role of Thrombospondin‑1 in sepsis‑induced myocardial injury

    DOI: 10.3892/mmr.2021.12509

    Sample: myocardial primary cell
  16. Natural Product Communications (2022) IF: 1.496
    Phillyrin Inhibits Isoproterenol-Induced Cardiac Hypertrophy Via P38 and NF-κB Pathways

    DOI: 10.1177/1934578X221144581

    Sample: serum
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