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Mouse OC/BGP(Osteocalcin) ELISA Kit

Citations(34)Uniprot : P86546

  • Cat.No.:E-EL-M0864

  • Reactivity: Mouse
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  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.39-25 ng/mL
Sensitivity 0.23 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse OC/BGP in samples. No significant cross-reactivity or interference between Mouse OC/BGP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse OC/BGP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse OC/BGP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse OC/BGP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse OC/BGP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse OC/BGP. You can calculate the concentration of Mouse OC/BGP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse OC/BGP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse OC/BGP were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.30 4.00 12.40 1.40 3.80 13.50
Standard deviation 0.10 0.20 0.50 0.10 0.20 0.50
CV (%) 7.69 5.00 4.03 7.14 5.26 3.70

Recovery

The recovery of Mouse OC/BGP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-105 100
EDTA plasma (n=8) 88-103 94
Cell culture media (n=8) 88-101 93

Linearity

Samples were spiked with high concentrations of Mouse OC/BGP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P86546
SynonymsBGLAP, OT, Bone Gamma-carboxyglutamate Protein, Bone Gla Protein
Research AreaCancer, Signal transduction, Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Advanced Science (2021) IF: 16.806
    Neuronal Induction of Bone-Fat Imbalance through Osteocyte Neuropeptide Y

    DOI: 10.1002/advs.202100808

    PMID: 34719888

    		 Sample: serum
  2. Advanced Science (2021) IF: 16.806
    Extracellular Vesicles from Child Gut Microbiota Enter into Bone to Preserve Bone Mass and Strength

    DOI: 10.1002/advs.202004831

    PMID: 33977075

    		 Sample: Serum
  3. Nature Communications (2022) IF: 14.919
    Aged bone matrix-derived extracellular vesicles as a messenger for calcification paradox

    DOI: 10.1038/s41467-022-29191-x

    PMID: 35304471

    		 Sample: Serum
  4. DEVELOPMENTAL CELL (2022) IF: 13.417
    Gasdermin D maintains bone mass by rewiring the endo-lysosomal pathway of osteoclastic bone resorption

    DOI: 10.1016/j.devcel.2022.09.013

    PMID: 36243012

    		 Sample: serum,osteoclasts
  5. Bone Research (2019) IF: 13.567
    Extracellular vesicles from human urine-derived stem cells prevent osteoporosis by transferring CTHRC1 and OPG

    DOI: 10.1038/s41413-019-0056-9
    Sample: serum
  6. Autophagy (2020) IF: 9.77
    Autophagy receptor OPTN (optineurin) regulates mesenchymal stem cell fate and bone-fat balance during aging by clearing FABP3

    DOI: 10.1080/15548627.2020.1839286

    PMID: 33143524

    		 Sample: Serum
  7. FREE RADICAL BIOLOGY AND MEDICINE (2022) IF: 8.1010
    A novel BMP2 secretagogue ameliorates glucocorticoid induced oxidative stress in osteoblasts by activating NRF2 dependent survival while promoting Wnt/β-catenin mediated osteogenesis

    DOI: 10.1016/j.freeradbiomed.2022.08.007

    PMID: 35963563

    		 Sample: serum
  8. CELL DEATH AND DIFFERENTIATION (2016) IF: 8.218
    IL-12p40 impairs mesenchymal stem cell-mediated bone regeneration via CD4 + T cells

    DOI: 10.1038/cdd.2016.72
    Sample: Serum
  9. Theranostics (2020) IF: 8.5790
    IL-23, but not IL-12, plays a critical role in inflammation-mediated bone disorders

    DOI: 10.7150/thno.41378
    Sample: serum
  10. Theranostics (2020) IF: 8.5790
    Human umbilical cord mesenchymal stromal cells-derived extracellular vesicles exert potent bone protective effects by CLEC11A-mediated regulation of bone metabolism

    DOI: 10.7150/thno.39238
    Sample: serum
  11. MOLECULAR THERAPY (2020) IF: 8.9860
    CD39 Produced from Human GMSCs Regulates the Balance of Osteoclasts and Osteoblasts through the Wnt/β-Catenin Pathway in Osteoporosis

    DOI: 10.1016/j.ymthe.2020.04.003
    Sample: serum,plasma
  12. BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 7.419
    Psoralen suppresses the phosphorylation of amyloid precursor protein (APP) to inhibit myelosuppression

    DOI: 10.1016/j.biopha.2022.113381

    PMID: 36076476

    		 Sample: MSCs
  13. BRITISH JOURNAL OF PHARMACOLOGY (2020) IF: 7.73
    Melatonin up‐regulates bone marrow mesenchymal stem cells osteogenic action but suppresses their mediated osteoclastogenesis via MT2‐inactivated NF‐κB pathway

    DOI: 10.1111/bph.14972
    Sample: Bone marrow mesenchymal stem cell
  14. JOURNAL OF NUTRITIONAL BIOCHEMISTRY (2022) IF: 6.117
    Maternal omega-3 LC-PUFA supplementation programs an improved bone mass in the offspring with a more pronounced effect in females than males at adulthood

    DOI: 10.1016/j.jnutbio.2022.109245
    Sample: plasma
  15. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2021) IF: 5.279
    PINK1/Parkin-Mediated Mitophagy Plays a Protective Role in the Bone Impairment Caused by Aluminum Exposure

    DOI: 10.1021/acs.jafc.1c01921

    PMID: 34018397

    		 Sample: Serum
  16. PHYTOMEDICINE (2022) IF: 5.34
    Activation of farnesoid X receptor signaling by geniposidic acid promotes osteogenesis

    DOI: 10.1016/j.phymed.2022.154258

    PMID: 35716540

    		 Sample: serum
  17. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
    Aromatic Bis[aminomethylidenebis(phosphonic)] Acids Prevent Ovariectomy-Induced Bone Loss and Suppress Osteoclastogenesis in Mice

    DOI: 10.3390/ijms22179590

    PMID: 34502499

    		 Sample: plasma
  18. MOLECULAR AND CELLULAR ENDOCRINOLOGY (2021) IF: 4.102
    Deficiency of Omentin-1 leads to delayed fracture healing through excessive inflammation and reduced CD31hiEmcnhi vessels

    DOI: 10.1016/j.mce.2021.111373
    Sample: serum
  19. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2019) IF: 4.183
    Methoxsalen and Bergapten Prevent Diabetes-Induced Osteoporosis by the Suppression of Osteoclastogenic Gene Expression in Mice

    DOI: 10.3390/ijms20061298

    PMID: 30875838

    		 Sample: Serum
  20. BONE (2019) IF: 4.36
    Blocking glucocorticoid signaling in osteoblasts and osteocytes prevents mechanical unloading-induced cortical bone loss

    DOI: 10.1016/j.bone.2019.115108
    Sample: serum
  21. BONE (2021) IF: 4.398
    Coupling Induction of Osteogenesis and Type H Vessels by Pulsed Electromagnetic Fields in Ovariectomy-induced Osteoporosis in Mice

    DOI: 10.1016/j.bone.2021.116211

    PMID: 34560308

    		 Sample: serum
  22. EUROPEAN JOURNAL OF PHARMACOLOGY (2022) IF: 4.432
    HDAC inhibitor quisinostat prevents estrogen deficiency-induced bone loss by suppressing bone resorption and promoting bone formation in mice

    DOI: 10.1016/j.ejphar.2022.175073

    PMID: 35636521

    		 Sample: serum
  23. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020) IF: 4.556
    Inflammatory Skin-Derived Cytokines Accelerate Osteoporosis in Mice with Persistent Skin Inflammation

    DOI: 10.3390/ijms21103620
    Sample: serum
  24. Disease Models & Mechanisms (2020) IF: 4.651
    Akkermansia muciniphila promotes type H vessels formation and bone fracture healing by reducing gut permeability and inflammation

    DOI: 10.1242/dmm.043620
    Sample: serum
  25. Materials Science & Engineering C-Materials for Biological Applications (2019) IF: 4.959
    Improving in vitro biocompatibility on biomimetic mineralized collagen bone materials modified with hyaluronic acid oligosaccharide

    DOI: 10.1016/j.msec.2019.110008

    PMID: 31499961

    		 Sample: Cell sample
  26. CHEMICO-BIOLOGICAL INTERACTIONS (2017) IF: 3.143
    Methoxsalen supplementation attenuates bone loss and inflammatory response in ovariectomized mice

    DOI: 10.1016/j.cbi.2017.10.014
    Sample: Serum
  27. BIOMEDICINE & PHARMACOTHERAPY (2018) IF: 3.457
    Beneficial effects of hyperoside on bone metabolism in ovariectomized mice

    DOI: 10.1016/j.biopha.2018.08.069
    Sample: serum
  28. Biomimetics (2022) IF: 3.743
    Osteoblast-like Cell Differentiation on 3D-Printed Scaffolds Using Various Concentrations of Tetra-Polymers

    DOI: 10.3390/biomimetics7020070

    PMID: 35735586

    		 Sample: MG3T3-E1 cell
  29. PROCESS BIOCHEMISTRY (2021) IF: 3.757
    Ferulic acid-Cu(II) and Zn(II) complexes promote bone formation

    DOI: 10.1016/j.procbio.2021.05.016
    Sample: Cell culture supernatant
  30. Scientific Reports (2020) IF: 3.998
    Disordered metabolism in mice lacking irisin

    DOI: 10.1038/s41598-020-74588-7

    PMID: 33060792

    		 Sample: Serum
  31. JOURNAL OF BONE AND MINERAL METABOLISM (2021) IF: 2.626
    Protective effects of berberine on senile osteoporosis in mice

    DOI: 10.1007/s00774-021-01225-2

    PMID: 33829324

    		 Sample: Serum
  32. INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED (2022) IF: 2.687
    MicroRNA-455–3p promotes osteoblast differentiation via targeting HDAC2

    DOI: 10.1016/j.injury.2022.08.047
    Sample: MC3T3-E1 cell
  33. Chemical Biology & Drug Design (2021) IF: 2.817
    Alginate oligosaccharide alleviates senile osteoporosis via the RANKL-RANK pathway in D-galactose-induced C57BL/6J mice

    DOI: 10.1111/cbdd.13904

    PMID: 34145772

    		 Sample: serum
  34. Journal of Ayurveda and Integrative Medicine (2017)
    Exploring molecular mechanism of bone-forming capacity of Eurycoma longifolia: Evidence of enhanced expression of bone-related biomarkers

    DOI: 10.1016/j.jaim.2017.04.005
    Sample: calvariae origin osteoblastic cell line subclone 4
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