Mouse Pro-ANP (Pro Atrial Natriuretic Peptide) CLIA Kit (E-CL-M0567)

Name: Mouse Pro-ANP (Pro Atrial Natriuretic Peptide) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0567
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Mouse Pro-ANP (Pro Atrial Natriuretic Peptide) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse Pro-ANP in samples.No significant cross-reactivity or interference between Mouse Pro-ANP and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	54897.000	57699.000	56298.000	56263
250	20690.000	21756.000	21223.000	21188
125	10105.000	9747.000	9926.000	9891
62.5	4447.000	4643.000	4545.000	4510
31.25	2564.000	2402.000	2483.000	2448
15.63	1388.000	1272.000	1330.000	1295
7.81	736.000	754.000	745.000	710
0	34.000	36.000	35.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	27.380	69.710	174.740	28.510	69.350	171.110
Standard deviation	2.980	6.950	13.930	3.230	8.220	10.850
CV（%）	10.880	9.970	7.970	11.330	11.850	6.340

Recovery

The recovery of Mouse Pro-ANP was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-105	96
EDTA plasma （n=8）	89-100	95
Cell culture media （n=8）	93-108	98

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse Pro-ANP and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	84-96	96-107	99-115
1:2	Average	91	101	107
1:4	Range	98-114	94-108	89-100
1:4	Average	105	99	94
1:8	Range	102-114	85-98	89-103
1:8	Average	108	92	97

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse Pro-ANP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Pro-ANP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Pro-ANP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse Pro-ANP. You can calculate the concentration of Mouse Pro-ANP in the samples by comparing the RLU value of the samples to the standard curve.

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