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Mouse TSH(Thyroid Stimulating Hormone) ELISA Kit

Cat.No.:E-EL-M1153
Reactivity: Mouse

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96T
48T
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	1.25-80 ng/mL
Sensitivity	0.75 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse TSH in samples. No significant cross-reactivity or interference between Mouse TSH and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse TSH concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TSH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TSH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TSH. You can calculate the concentration of Mouse TSH in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse TSH were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse TSH were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	4.40	7.30	36.90	4.40	7.10	39.00
Standard deviation	0.30	0.30	1.70	0.30	0.40	1.20
CV (%)	6.82	4.11	4.61	6.82	5.63	3.08

Recovery

The recovery of Mouse TSH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	88-98	93
EDTA plasma (n=8)	95-107	100
Cell culture media (n=8)	91-106	99

Linearity

Samples were spiked with high concentrations of Mouse TSH and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	Thyrotropin
Research Area	Cancer, Metabolism, Neuroscience, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Small Methods (2017) IF: 14.188
Transdermal Delivery of Anti‐Obesity Compounds to Subcutaneous Adipose Tissue with Polymeric Microneedle Patches

DOI: 10.1002/smtd.201700269

Sample: Serum
PHARMACOLOGICAL RESEARCH (2023) IF: 10.334
Repositioning of Cefuroxime as novel selective inhibitor of the thyroid hormone activating enzyme type 2 Deiodinase

DOI: 10.1016/j.phrs.2023.106685

Sample: Blood
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
Transcriptome Profile of Thyroid Glands in Bile Duct Ligation Mouse Model

DOI: 10.3390/ijms23158244

PMID: 35897811
Sample: serum
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2019) IF: 6.291
Chronic exposure to microcystin-LR reduces thyroid hormone levels by activating p38/MAPK and MEK/ERK signal pathway

DOI: 10.1016/j.ecoenv.2019.02.024

PMID: 30771657
Sample: Plasma
Oxidative Medicine and Cellular Longevity (2021) IF: 6.543
TSH Combined with TSHR Aggravates Diabetic Peripheral Neuropathy by Promoting Oxidative Stress and Apoptosis in Schwann Cells

DOI: 10.1155/2021/2482453

PMID: 34804362
Sample: plasma
THYROID (2020) IF: 5.309
The Thyroid Hormone Inactivator Enzyme, Type 3 Deiodinase, Is Essential for Coordination of Keratinocyte Growth and Differentiation

DOI: 10.1089/thy.2019.0557

PMID: 32111151
Sample: Cell culture supernatant
Journal of Immunology Research (2022) IF: 4.493
Saikosaponin-d Attenuates Hashimoto’s Thyroiditis by Regulating Macrophage Polarization

DOI: 10.1155/2022/7455494

Sample: blood
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2020) IF: 3.056
Renal protective effects of grape seed extract treatment against Eltroxin-induced hyperthyroidism, kidney damage, and oxidative stress in male mice

DOI: 10.1007/s11356-020-08210-8

Sample: serum
Reproductive Biology and Endocrinology (2019) IF: 2.589
Reduced expression of PD-L1 in autoimmune thyroiditis attenuate trophoblast invasion through ERK/MMP pathway

DOI: 10.1186/s12958-019-0536-1

PMID: 31656199
Sample: Tissue homogenate
JOURNAL OF FOOD BIOCHEMISTRY (2021) IF: 2.72
Subclinical hypothyroidism contributes to poor glycemic control in patients with type 2 diabetes mellitus, and ellagic acid attenuates methimazole-induced abnormal glucose metabolism in mice model

DOI: 10.1111/jfbc.13753

PMID: 33955004
Sample: Serum

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