Mouse TTR (Transthyretin) CLIA Kit (E-CL-M0664)

Name: Mouse TTR (Transthyretin) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0664
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.94 ng/mL
Detection Range	1.56-100 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse TTR in samples.No significant cross-reactivity or interference between Mouse TTR and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
100	36563.000	38689.000	37626.000	37604
50	15962.000	19350.000	17656.000	17634
25	8028.000	7070.000	7549.000	7527
12.5	3525.000	4173.000	3849.000	3827
6.25	2026.000	1748.000	1887.000	1865
3.13	1107.000	969.000	1038.000	1016
1.56	610.000	614.000	612.000	590
0	22.000	22.000	22.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	4.810	8.740	35.150	4.420	8.490	37.810
Standard deviation	0.520	1.040	2.580	0.430	0.890	3.550
CV（%）	10.810	11.900	7.340	9.730	10.480	9.390

Recovery

The recovery of Mouse TTR was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	86-101	92
EDTA plasma （n=8）	99-112	104
Cell culture media （n=8）	98-112	105

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse TTR and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	101-118	92-104	100-116
1:2	Average	107	97	108
1:4	Range	89-102	88-99	95-112
1:4	Average	95	93	102
1:8	Range	91-105	90-100	90-104
1:8	Average	96	95	96

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TTR. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TTR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TTR, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse TTR. You can calculate the concentration of Mouse TTR in the samples by comparing the RLU value of the samples to the standard curve.

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