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Mouse TXB2(Thromboxane B2) ELISA Kit

Cat.No.:E-EL-M1144
Reactivity: Mouse

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96T
48T
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96T*5
96T*10

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Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T
Assay time	2.0h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 50μL
Specificity	This kit recognizes TXB2 in samples. No significant cross-reactivity or interference between TXB2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of TXB2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with TXB2. During the reaction, TXB2 in the sample or standard competes with a fixed amount of TXB2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to TXB2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TXB2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level TXB2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level TXB2 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	47.20	87.00	453.50	50.00	80.30	446.10
Standard deviation	3.30	5.00	19.00	2.70	3.80	14.30
CV (%)	6.99	5.75	4.19	5.40	4.73	3.21

Recovery

The recovery of TXB2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	85-97	91
EDTA plasma(n=8)	93-106	98
Cell culture media(n=8)	95-108	100

Linearity

Samples were spiked with high concentrations of Mouse TXB2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	TX-B2
Research Area	Metabolism, Immunology

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE (2015) IF: 12.996
Platelet Activation and Aggregation Promote Lung Inflammation and Influenza Virus Pathogenesis

DOI: 10.1164/rccm.201406-1031OC

Sample: Tissue homogenate
Theranostics (2019) IF: 8.0630
Temporal inhibition of mouse mammary gland cancer metastasis by CORM-A1 and DETA/NO combination therapy

DOI: 10.7150/thno.31461

Sample: plasma
Frontiers in Immunology (2018) IF: 5.511
Microthrombotic Renal Vascular Lesions Are Associated to Increased Renal Inflammatory Infiltration in Murine Lupus Nephritis

DOI: 10.3389/fimmu.2018.01948

PMID: 30210500
Sample: Serum
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
Platelet Inhibition by Low-Dose Acetylsalicylic Acid Reduces Neuroinflammation in an Animal Model of Multiple Sclerosis

DOI: 10.3390/ijms22189915

PMID: 34576080
Sample: plasma
MOLECULAR PHARMACEUTICS (2018) IF: 4.556
Combination Therapy with DETA/NO and Clopidogrel Inhibits Metastasis in Murine Mammary Gland Cancer Models via Improved Vasoprotection

DOI: 10.1021/acs.molpharmaceut.8b00781

PMID: 30234992
Sample: Plasma,Tissue homogenate
Molecular Medicine Reports (2017) IF: 1.692
Protective effects of marrubiin improve endometriosis through suppression of the expression of RANTES

DOI: 10.3892/mmr.2017.6969

Sample: endometriotic cell-Xenograft

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