Mouse VEGF-C (Vascular Endothelial cell Growth Factor C) CLIA Kit (E-CL-M0679)

Name: Mouse VEGF-C (Vascular Endothelial cell Growth Factor C) CLIA Kit
Brand: Elabscience
SKU: E-CL-M0679
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Mouse VEGF-C (Vascular Endothelial cell Growth Factor C) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse VEGF-C in samples.No significant cross-reactivity or interference between Mouse VEGF-C and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	52462.000	53142.000	52802.000	52767
400	20338.000	24606.000	22472.000	22437
200	11305.000	9881.000	10593.000	10558
100	4477.000	5353.000	4915.000	4880
50	2489.000	2135.000	2312.000	2277
25	1193.000	1035.000	1114.000	1079
12.5	527.000	563.000	545.000	510
0	35.000	35.000	35.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	39.370	120.900	399.970	37.990	117.060	400.990
Standard deviation	4.650	13.790	27.520	4.110	12.030	33.200
CV（%）	11.810	11.410	6.880	10.820	10.280	8.280

Recovery

The recovery of Mouse VEGF-C was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	95-107	100
EDTA plasma （n=8）	89-104	97
Cell culture media （n=8）	85-95	90

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse VEGF-C and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	95-113	101-115	101-118
1:2	Average	103	107	109
1:4	Range	103-120	91-105	99-116
1:4	Average	109	96	107
1:8	Range	94-109	93-106	85-97
1:8	Average	101	99	90

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse VEGF-C. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse VEGF-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse VEGF-C, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse VEGF-C. You can calculate the concentration of Mouse VEGF-C in the samples by comparing the RLU value of the samples to the standard curve.

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