MTT is 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide which can be reduced by some dehydrogenases in mitochondria to form a crystalline dark purple product formazan. Formazan can be completely dissolved by DMSO and its absorbance is near 570 nm wavelength. The more and faster the cells proliferate, the darker the color is, and the more cytotoxic, the lighter the color is. For the same cells, there is a linear relationship between the depth darkness of color and the number of cells.

General Information

Application	Viability/Proliferation/Cytotoxicity
Detection method	Colorimetric Method
Sample type	Cell samples
Detection instrument	Microplate reader
Other reagents required	DMSO
Storage	2~8°C, shading light
Shipping	Ice bag
Expiration date	6 months

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Citations

MOLECULES (2022) IF: 4.927
Oncolytic Newcastle Disease Virus Co-Delivered with Modified PLGA Nanoparticles Encapsulating Temozolomide against Glioblastoma Cells: Developing an Effective Treatment Strategy

DOI: 10.3390/molecules27185757

PMID: 36144488
MOLECULES (2023) IF: 4.927
Enhanced Intracellular Photosensitizer Uptake and Retention by Targeting Viral Oncoproteins in Human Papillomavirus Infected Cancer Cells and Cancer Stem Cells

DOI: 10.3390/molecules28020647
FASEB JOURNAL (2020) IF: 4.966
CaMK4‐dependent phosphorylation of Akt/mTOR underlies Th17 excessive activation in experimental autoimmune prostatitis

DOI: 10.1096/fj.201902910RRR

Sample: Cell
Photodiagnosis and Photodynamic Therapy (2021) IF: 3.631
Influence of antibacterial effects of tetracycline, laser, and photodynamic therapy on cell viability, cell damage, and virulence of Porphyromonas gingivalis.

DOI: 10.1016/j.pdpdt.2021.102617
BMC Complementary and Alternative Medicine (2022) IF: 3.659
Evaluation of the antimalarial activity and toxicity of Mahanil-Tang-Thong formulation and its plant ingredients

DOI: 10.1186/s12906-022-03531-2

PMID: 35219319
SURFACE ENGINEERING (2020) IF: 2.433
Ag-containing antibacterial self-healing micro-arc oxidation coatings on Mg–Zn–Sr alloys

DOI: 10.1080/02670844.2020.1800346

Sample: Cell culture supernatant
NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL (2021) IF: 2.9
Catechin Metabolites along with Curcumin Inhibit Proliferation and Induce Apoptosis in Cervical Cancer Cells by Regulating VEGF Expression In-Vitro

DOI: 10.1080/01635581.2021.1936082

PMID: 34121550

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