Click here to view cell apoptosis assay products

NAD-Isocitrate Dehydrogenase (NAD-IDH) Activity Assay Kit

  • Cat.No.:E-BC-K651-M

  • Detection instrument: Microplate reader (440-460 nm, optimum wavelength: 450 nm)

To Purchase E-BC-K651-M

  • 96T
Price: $380

Welcome to order from local distributors.

Protocols for metabolism assays

FAQ for metabolism assays

Have Questions?

 Add to cart Bulk request

For research use only. Order now, ship in 3 days

Detection principle

Isocitrate dehydrogenase (IDH) is one of the inverters in the tricarboxylic acid cycle and plays an important role in energy metabolism, amino acid and vitamin synthesis. The cofactors of this enzyme include two kinds of NAD+ and NADP+, which are located in different parts of the cell respectively. In eukaryotic cells, NAD-dependent isocitrate dehydrogenase mainly exists in mitochondria. Under the activation of the activator, IDH converts isocitrate into α-ketoglutaric acid. Meanwhile, NAD+ is reduced to NADH, which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of NAD-IDH can be calculated by measuring the change of absorbance value at 450 nm.

Performance characteristics

Synonyms NAD-IDH
Sample type animal tissue
Sensitivity 0.84 U/L
Detection range 0.84-50 U/L
Assay type Enzyme Activity
Assay time 50 min
Precision Average inter-assay CV: 8.600%Average intra-assay CV: 5%
Other instruments required Centrifuge,37℃ incubator
Storage -20℃
Valid period 12 months



  • Show all
  • Reviews
  • Q&A
... Show All Show Less

People Also Bought

Apply for FREE TRIAL Fluorometric Assay kit
*Product Name:
*Catalog Number:
*When will you use it?