Nitric Oxide (NO) Colorimetric Assay Kit (E-BC-K035-S)
For research use only.
| Detection Principle | NO is easily oxidized to form NO2- in vivo or in aqueous solution, and a reddish azo compound is formed with the color developing agent, and the concentration of the azo compound is linearly related to the concentration of NO. The concentration of NO can be calculated indirectly by measuring the OD value at 550 nm. |
| Synonyms | NO |
| Sample Type | Serum,Plasma,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (550 nm) |
| Research Area | Oxidative Stress |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Valid Period | 12 months |
| Sensitivity | 0.97 μmol/L |
| Detection Range | 0.97-700 μmol/L |
| Precision | inter-assay CV: 5.2% | intra-assay CV: 3.4% |
| Sample Volume | 200-300 μL |
| Assay Time | 1 h 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human plasma | 1 |
| Rat serum | 1 |
| Rat plasma | 1 |
| 10% Mouse liver tissue homogenization | 1 |
| 10% Epipremnum aureum tissue homogenization | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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