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Nitric Oxide (NO) Colorimetric Assay Kit

Citations(29)

  • Cat.No.:E-BC-K035-S

  • Detection instrument: Spectrophotometer(550 nm)

Manual MSDS

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Detection principle

NO is easily oxidized to form NO2- in vivo or in aqueous solution, and a reddish azo compound is formed with the color developing agent, and the concentration of the azo compound is linearly related to the concentration of NO. The concentration of NO can be calculated indirectly by measuring the OD value at 550 nm.

Performance characteristics

Synonyms NO
Sample type Serum,plasma,tissue
Sensitivity 0.97 μmol/L
Detection range 0.97-700 μmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 80 min
Precision Average inter-assay CV: 5.200%Average intra-assay CV: 3.400%
Other instruments required Vortex mixer, Centrifuge, Analytical Balance, Micropipettor
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage Reagent 6: -20℃, others: 2-8℃
Valid period 12 months

Images

NO level was significantly reduced by cold exposure. (P<0.01)

BYWng et al investigate the mechanism between cold exposure, gut microbiota and hypertension. Nitric oxide (NO) level of rat plasma was determined using NO colorimetric assay kit (E-BC-K035-S).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.97-700 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Human plasma

1

10% Mouse liver tissue homogenization

1

Rat serum

1

Rat plasma

1

10% Epipremnum aureum tissue homogenization

1

Note: The diluent is double distilled water, normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

  1. CHEMOSPHERE (2022) IF: 7.086
    Developmental, toxicological effects and recovery patterns in Xenopus laevis after exposure to penconazole-based fungicide during the metamorphosis process

    DOI: 10.1016/j.chemosphere.2022.135302

    PMID: 35697111

    		 Sample: embryo
  2. Frontiers in Immunology (2021) IF: 7.561
    Transcriptional Profiling and Functional Analysis of N1/N2 Neutrophils Reveal an Immunomodulatory Effect of S100A9-Blockade on the Pro-Inflammatory N1 Subpopulation

    DOI: 10.3389/fimmu.2021.708770
    Sample: neutrophils
  3. FOOD AND CHEMICAL TOXICOLOGY (2021) IF: 6.025
    Reactive astrocytes induced by 2-chloroethanol modulate microglia polarization through IL-1β, TNF-α, and iNOS upregulation

    DOI: 10.1016/j.fct.2021.112550

    PMID: 34517076

    		 Sample: astrocyte
  4. DRUG DELIVERY (2022) IF: 6.419
    Silk fibroin hydrogel containing Sesbania sesban L. extract for rheumatoid arthritis treatment

    DOI: 10.1080/10717544.2022.2050848

    PMID: 35277106

    		 Sample: RAW 264.7 cell
  5. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    D-ribose-L-cysteine prevents oxidative stress and cardiometabolic syndrome in high fructose high fat diet fed rats

    DOI: 10.1016/j.biopha.2021.112017

    PMID: 34399203

    		 Sample: heart
  6. HYPERTENSION (2018) IF: 6.823
    Potential Protective Mechanism in the Cardiac Microvascular Injury

    DOI: 10.1161/HYPERTENSIONAHA.118.11035
    Sample: cardiac microvascular endothelial cells (CMECs)
  7. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021) IF: 6.953
    Structural characterization and immunomodulatory activities of two polysaccharides from Rehmanniae Radix Praeparata

    DOI: 10.1016/j.ijbiomac.2021.06.100

    PMID: 34197855

    		 Sample: RAW264.7 cells
  8. Frontiers in Pharmacology (2021) IF: 5.811
    Shaoyao-Gancao Decoction Ameliorates the Inflammation State in Polycystic Ovary Syndrome Rats via Remodeling Gut Microbiota and Suppressing the TLR4/NF-κB Pathway

    DOI: 10.3389/fphar.2021.670054

    PMID: 34054541

    		 Sample: Cell culture supernatant
  9. Frontiers in Pharmacology (2022) IF: 5.811
    Effects of Pereskia aculeate Miller Petroleum Ether Extract on Complete Freund’s Adjuvant-Induced Rheumatoid Arthritis in Rats and its Potential Molecular Mechanisms

    DOI: 10.3389/fphar.2022.869810
    Sample: RAW264.7
  10. ACS Omega (2022) IF: 4.132
    Ajuga bracteosa Exerts Antihypertensive Activity in l-NAME-Induced Hypertension Possibly through Modulation of Oxidative Stress, Proinflammatory Cytokines, and the Nitric Oxide/Cyclic Guanosine Monophosphate Pathway

    DOI: 10.1021/acsomega.2c03888

    PMID: 36157749

    		 Sample: serum
  11. SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2022) IF: 4.219
    Evaluation of the antioxidative and genotoxic effects of sodium butyrate on breast cancer cells

    DOI: 10.1016/j.sjbs.2021.12.061

    PMID: 35280546

    		 Sample: MCF-7 cell
  12. Food Bioscience (2021) IF: 4.24
    Auricularia polytricha and Flammulina velutipes ameliorate inflammation and modulate the gut microbiota via regulation of NF-κB and Keap1/Nrf2 signaling pathways on DSS-induced inflammatory bowel disease

    DOI: 10.1016/j.fbio.2021.101426
    Sample: colon,liver
  13. JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
    Ziziphus Oxyphylla hydro-methanolic extract ameliorates hypertension in L-NAME induced hypertensive rats through NO/cGMP pathway and suppression of oxidative stress related inflammatory biomarkers.

    DOI: 10.1016/j.jep.2021.114825
    Sample: Serum
  14. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    Aronia melanocarpa (Michx.) Elliott. attenuates dextran sulfate sodium-induced Inflammatory Bowel Disease via regulation of inflammation-related signaling pathways and modulation of the gut microbiota

    DOI: 10.1016/j.jep.2022.115190

    PMID: 35306040

    		 Sample: liver homogenates
  15. Andrology (2022) IF: 4.456
    Icariin inhibits the formation of mitochondria-associated membranes (MAMs) and improves erectile function in rats treated with prostate radiation

    DOI: 10.1111/andr.13218

    PMID: 35765205

    		 Sample: penile cavernous tissue
  16. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
    Semen Brassicae reduces thoracic aortic remodeling, inflammation, and oxidative damage in spontaneously hypertensive rats

    DOI: 10.1016/j.biopha.2020.110400

    PMID: 32570115

    		 Sample: Cell sample
  17. MOLECULES (2023) IF: 4.927
    Neuroprotective Effects of Savinin on LPS-Induced Neuroinflammation In Vivo via Regulating MAPK/NF-κB Pathway and NLRP3 Inflammasome Activation

    DOI: 10.3390/molecules28041575
    Sample: hippocampa
  18. JOURNAL OF ETHNOPHARMACOLOGY (2018) IF: 3.115
    Anti-inflammatory action of Athyrium multidentatum extract suppresses the LPS-induced TLR4 signaling pathway

    DOI: 10.1016/j.jep.2018.02.031
    Sample: peritoneal macrophages (PMs)
  19. Animals (2022) IF: 3.231
    Milk Pathogens in Correlation with Inflammatory, Oxidative and Nitrosative Stress Markers in Goat Subclinical Mastitis

    DOI: 10.3390/ani12233245
    Sample: skimmed milk
  20. Animals (2022) IF: 3.231
    Expression of Hormones, Cytokines, and Antioxidants in Heat-Stressed Subfertile Female Dromedaries

    DOI: 10.3390/ani12162125

    PMID: 36009715

    		 Sample: serum
  21. Andrology (2022) IF: 3.842
    Low androgen status inhibits erectile function by upregulating the expression of proteins of mitochondria-associated membranes in rat corpus cavernosum

    DOI: 10.1111/andr.13188

    PMID: 35466583

    		 Sample: penile cavernous tissue
  22. Molecular Medicine Reports (2020) IF: 2.1
    Penehyclidine hydrochloride alleviates lipopolysaccharide‑induced acute respiratory distress syndrome in cells via regulating autophagy‑related pathway

    DOI: 10.3892/mmr.2020.11739

    PMID: 33300058

    		 Sample: Cell culture supernatant
  23. Experimental and Therapeutic Medicine (2021) IF: 2.447
    Pitavastatin maintains MAPK7 expression and alleviates angiotensin II‑induced vascular endothelial cell inflammation and injury

    DOI: 10.3892/etm.2021.11055
    Sample: HUVEC
  24. SAUDI PHARMACEUTICAL JOURNAL (2020) IF: 2.879
    Radix pseudostellariae of Danzhi Jiangtang capsule relieves oxidative stress of vascular endothelium in diabetic macroangiopathy

    DOI: 10.1016/j.jsps.2020.04.009

    PMID: 32550799

    		 Sample: Serum
  25. MOLECULAR BIOLOGY REPORTS (2020) IF: 1.402
    Codeine-induced hepatic injury is via oxido-inflammatory damage and caspase-3-mediated apoptosis

    DOI: 10.1007/s11033-020-05983-6

    PMID: 33211294

    		 Sample: lvier
  26. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL (2019) IF: 1.645
    Adventitious root cultures of Oplopanax elatus inhibit LPS-induced inflammation via suppressing MAPK and NF-κB signaling pathways

    DOI: 10.1007/s11626-019-00396-7
    Sample: PMs cells
  27. Journal of Clinical Tuberculosis and Other Mycobacterial Diseases (2021)
    Phagocytosis and the antigen-processing abilities of macrophages derived from monocytes in spinal tuberculosis patients

    DOI: 10.1016/j.jctube.2021.100215
    Sample: macrophages
  28. Advances in Traditional Medicine (2020)
    Anti-hypernociceptive effects of methanol extract of Boswellia dalzielii on STZ-induced diabetic neuropathic pain

    DOI: 10.1007/s13596-019-00411-y
    Sample: serum,sciatic nerves
  29. Natural Product Communications (2020) IF: 0.468
    Regulation of PI3k-WNK Pathway by Ethyl Acetate Partition Fraction of Gardenia jasminoides var. radicans Makino in SHR, NRK52e Cells, and IMCD3 Cells:

    DOI: 10.1177/1934578X20920989
    Sample: Serum
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Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
Q

J****eSubmitted [ Mar 10 2023 ]

Asked: Can this kit use for NO measurement in oil (not water solvent)?

Reply

A

adminSubmitted [ Mar 10 2023 ]

Answered: Sorry, this kit can not use for oil.

Q

M*****iSubmitted [ Sep 08 2022 ]

Asked: How long can the chromogenic reagent be stored after it is prepared？

Reply

A

adminSubmitted [ Sep 08 2022 ]

Answered: It is recommended to use it within half a day

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