Nitric Oxide (NO) Colorimetric Assay Kit

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K035-S

      Size:
      • 100Assays
      • 50Assays
      Qty:
      - +
      Price: $150
      Can detect 96 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(550 nm)

      Lead Time: Order now, ship in 3 daysWelcome to order from local distributors.

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      Detection principle

      NO is easily oxidized to form NO2- in vivo or in aqueous solution, and a reddish azo compound is formed with the color developing agent, and the concentration of the azo compound is linearly related to the concentration of NO. The concentration of NO can be calculated indirectly by measuring the OD value at 550 nm.

      Performance characteristics

      Synonyms NO
      Sample type Serum,plasma,tissue
      Sensitivity 0.97 μmol/L
      Detection range 0.97-700 μmol/L
      Detection method Colorimetric method
      Assay type Quantitative
      Assay time 80 min
      Precision Average inter-assay CV: 5.2%Average intra-assay CV: 3.4%
      Other instruments required Vortex mixer, Centrifuge, Analytical Balance, Micropipettor
      Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
      Storage 2-8℃
      Valid period 6 months

      Citations

      1. Antihypernociceptive and neuroprotective effects of Combretin A and Combretin B on streptozotocin-induced diabetic neuropathy in mice
        IF: 2.058
        Journal: Naunyn-Schmiedeberg's Archives of Pharmacology(2019)
        DOI: 10.1007/s00210-019-01626-1
        PMID: 30759263
        Products: E-BC-K025-S E-BC-K035-S E-BC-K019-S E-BC-K031-S 
        Sample type: Serum,Tissue homegenate
        Reactivity: Mouse
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      MSDS for ELISA ISO 9001

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