English Toll-free:1-888-852-8623 or Contact Us
Keyword cannot be empty !

One-step TUNEL Flow Cytometry Apoptosis Kit (Red, Elab Fluor® 647)

Cat:E-CK-A424
Manual MSDS

Price: $ 498

Price: $ 298

Price: $ 198

Size:
100Assays 50Assays 20Assays
Quantity:
  • Kit Type: Apoptosis/DNA Fragmentation
Add to cart

For research use only. Order now, ship in 3 days

Product Details
Detection Principle When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with flow cytometry.
Detection Method Fluorometric Method;Flow Cytometry
Sample Type Cell samples
Assay Time 2.5-3 hours
Detection Instrument Flow Cytometer
Dye Type Elab Fluor® 647
Ex/Em 650/670
Channel Set APC
Other Reagents Required PBS buffer (with 1%BSA) (pH7.2~7.4)
Storage This product can be stored at -20°C for 12 months with shading light.
Expiration date 12 months
Shipping Ice bag
  • Q1:Can TUNEL assay kit be used for mouse samples?

    There is no species limination in TUNEL assay kits.

  • Q2:What are the reasons that lead to false positives in TUNEL?

    Cells with high proliferative or metabolic active may have a break in the DNA strand, both cases may end up with false-positive result. Evaluation of cell morphology may be used if there are doubts about the interpretation of the results.

  • Q3:Can the One-step TUNEL kit detect a sperm sample?

    Yes, it can be used to test sperm samples.

  • Q4:What is the difference between TUNEL in-situ kit and TUNEL flow cytometry kit?

    The main difference lies in the detection methods, for TUNEL in-situ kits, a fluorescence microscope is used for observing the results, as for TUNEL flow ytometry kit, the sample will be detected by a flow cytometer. On the other hand, TUNEL in-situ kit is more suitable for tissue sections and cell slides or smears, while TUNEL flow ytometry kit is more suitable for suspended cells and adherent cells.

  • Q5:Is there any TUNEL assay kit that can detect apoptosis in erythrocyte?

    The detection of TUNEL is based on DNA fragmentation in late stage apoptosis, so it is not avaliable for erythrocyte as there is no nuclei or other organelles containing DNA in erythrocyte.