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One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor^® 594)

Cat.No.:E-CK-A322

Applications:Apoptosis/DNA Fragmentation

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Detection principle

When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.

General Information

Application	DNA Fragmentation
Detection method	Fluorometric Method
Sample type	Paraffin section, frozen section, cell slide
Assay time	3 hours
Detection instrument	Fluorescence Microscope
Dye Type	Elab Fluor^® 594
Ex/Em (nm)	590/617
Filter set	TRITC
Other reagents required	4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-IR-R187),ddH2O,Mounting solution with anti-fluorescence quencher(E-IR-R119),Xylene,Ethanol
Storage	-20°C, shading light
Shipping	Ice bag
Expiration date	12 months

Related Products

E-CK-A321	One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor^® 488)
E-CK-A211	Annexin V-FITC/PI Apoptosis Kit
E-CK-A301	Mitochondrial Membrane Potential Assay Kit (with JC-1)
E-CK-A362	Enhanced Cell Counting Kit 8 (WST-8/CCK8)

Operation Guide

Citations

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