When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.
|Detection method||Fluorometric Method|
|Sample type||Paraffin section, frozen section, cell slide|
|Assay time||3 hours|
|Detection instrument||Fluorescence Microscope|
|Dye Type||Elab Fluor® 594|
|Other reagents required||4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-IR-R187),ddH2O,Mounting solution with anti-fluorescence quencher(E-IR-R119),Xylene,Ethanol|
|Storage||-20°C, shading light|
|Expiration date||12 months|
|E-CK-A321||One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488)|
|E-CK-A211||Annexin V-FITC/PI Apoptosis Kit|
|E-CK-A301||Mitochondrial Membrane Potential Assay Kit (with JC-1)|
|E-CK-A362||Enhanced Cell Counting Kit 8 (WST-8/CCK8)|
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|Sample: Tissue Slice|