Current Position:Home>>Cell Products>>Cell Apoptosis Assay>>TUNEL Assay Kit>> One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 594)
Click to find the fluorescence fit you.
Welcome to order from local distributors.
Add to cart Bulk request
For research use only. Order now, ship in 3 days
When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.
|Detection method||Fluorometric Method|
|Sample type||Paraffin section, frozen section, cell slide|
|Assay time||3 hours|
|Detection instrument||Fluorescence Microscope|
|Dye Type||Elab Fluor® 594|
|Other reagents required||4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-IR-R187),ddH2O,Mounting solution with anti-fluorescence quencher(E-IR-R119),Xylene,Ethanol|
|Storage||-20°C, shading light|
|Expiration date||12 months|
|E-CK-A321||One-step TUNEL In Situ Apoptosis Kit (Green, Elab Fluor® 488)|
|E-CK-A211||Annexin V-FITC/PI Apoptosis Kit|
|E-CK-A301||Mitochondrial Membrane Potential Assay Kit (with JC-1)|
|E-CK-A362||Enhanced Cell Counting Kit 8 (WST-8/CCK8)|
From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to firstname.lastname@example.org, we will get back to you with the reward after we confirm it ASAP!
Subtotal：$View / Edit