Alternate Names	B7-H1, PD-L1,Programmed cell death ligand 1,B7 homolog 1,B7-H, B7H1, PDL1, PDCD1L1, PDCD1LG1
Swissprot	Q9EP73
References	1. Sharpe A, et al. 2007. Nat. Immunol. 8:239. 2. Dong H, et al. 1999. Nat. Med. 5:1365. 3. Freeman G, et al. 2000. J. Exp. Med. 192:1030.

Product Details

Form	Liquid
Clone No.	10F.9G2(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2b, κ
Isotype Control	PE Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09842D]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PE
	PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T cell proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cell proliferation and cytokine production.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse CD274/PD-L1 Antibody[10F.9G2]	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse CD274/PD-L1 Antibody[10F.9G2]	25μg, 100μg	Check More
Biotin Anti-Mouse CD274/PD-L1 Antibody[10F.9G2]	25μg, 100μg	Check More
FITC Anti-Mouse CD274/PD-L1 Antibody[10F.9G2]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD274/PD-L1 Antibody[10F.9G2]	50Tests, 100Tests, 100Tests×2	Check More

Elab Fluor^® 488 Anti-Mouse CD274/PD-L1 Antibody[10F.9G2]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD274/PD-L1 Antibody[10F.9G2]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD274/PD-L1 Antibody[10F.9G2]	25μg, 100μg	Check More
PE Anti-Mouse CD274/PD-L1 Antibody[10F.9G2]	25μg, 100μg	Check More
APC Anti-Mouse CD274/PD-L1 Antibody[10F.9G2]	25μg, 100μg	Check More

Elab Fluor^® 488 Anti-Mouse CD274/PD-L1 Antibody[10F.9G2]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD274/PD-L1 Antibody[10F.9G2]	25μg, 100μg	Check More

Citations

Acta Pharmaceutica Sinica B (2022) IF: 14.903
Anti-PD-L1 antibody enhances curative effect of cryoablation via antibody-dependent cell-mediated cytotoxicity mediating PD-L1highCD11b+ cells elimination in hepatocellular carcinoma

DOI: 10.1016/j.apsb.2022.08.006

Sample: NK cell,cDC1 cell

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