Alternate Names	T-lymphocyte activation antigen CD86,Cd86,Activation B7-2 antigen,Early T-cell costimulatory molecule 1,ETC-1
Swissprot	P42082
References	1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Hathcock KS, et al. 1993. Science 262:905. 3. Freeman GJ, et al. 1993. Science 262:907. 4. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.

Product Details

Form	Liquid
Clone No.	GL-1(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2a, κ
Isotype Control	PE/Cyanine5.5 Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09832I]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PE/Cyanine5.5
	PE/Cyanine5.5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 690 nm (e.g., a 690/50 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse CD86 Antibody[GL-1]	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
Biotin Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
FITC Anti-Mouse CD86 Antibody[GL-1]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD86 Antibody[GL-1]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD86 Antibody[GL-1]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD86 Antibody[GL-1]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD86 Antibody[GL-1]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD86 Antibody[GL-1]	50Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD86 Antibody[GL-1]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
PE Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
APC Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More
PE/Elab Fluor^® 594 Anti-Mouse CD86 Antibody[GL-1]	25μg, 100μg	Check More

Citations

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