Alternate Names	T-cell surface glycoprotein CD8 alpha chain,CD8A,T-lymphocyte differentiation antigen T8/Leu-2,MAL
Swissprot	P07725
References	1. Johnson P, et al. 1985. EMBO J. 4:2539. 2. Thomas ML, et al. 1983. Eur. J. Immunol. 13:855.

Product Details

Form	Liquid
Clone No.	OX-8(See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG1, κ
Isotype Control	PE/Cyanine5.5 Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09793I]
Reactivity	Rat
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10⁶ cells in 100 μL volume].
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PE/Cyanine5.5
	PE/Cyanine5.5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 690 nm (e.g., a 690/50 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD8a is a 32 kD glycoprotein also known as T8, Lyt2, Ly-2, and CD8α. CD8a is a member of the immunoglobulin superfamily expressed on most thymocytes, subset of mature T cells, most NK cells, macrophages, and some activated CD4+ T cells (not resting). CD8a forms heterodimers with the CD8β chain (CD8b) on the surface of most thymocytes, while mature peripheral T lymphocytes express almost exclusively the CD8 αβ heterodimer. Intestinal intraepithelial lymphocytes express CD8a without CD8b. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
FITC Anti-Rat CD8a Antibody[OX-8]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Rat CD8a Antibody[OX-8]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Rat CD8a Antibody[OX-8]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Rat CD8a Antibody[OX-8]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Rat CD8a Antibody[OX-8]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Rat CD8a Antibody[OX-8]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Rat CD8a Antibody[OX-8]	25μg, 100μg	Check More
PE Anti-Rat CD8a Antibody[OX-8]	25μg, 100μg	Check More
APC Anti-Rat CD8a Antibody[OX-8]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Rat CD8a Antibody[OX-8]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Rat CD8a Antibody[OX-8]	25μg, 100μg	Check More

Citations

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