Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> PE/Cyanine5.5 Anti-Rat CD8a Antibody[OX-8]
Cat.No.:E-AB-F1098UI
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Alternate Names | T-cell surface glycoprotein CD8 alpha chain,CD8A,T-lymphocyte differentiation antigen T8/Leu-2,MAL |
Swissprot | P07725 |
References |
1. Johnson P, et al. 1985. EMBO J. 4:2539.
2. Thomas ML, et al. 1983. Eur. J. Immunol. 13:855. |
Form | Liquid |
Clone No. | OX-8(See Other Available Formats) |
Host | Mouse |
Isotype | Mouse IgG1, κ |
Isotype Control | PE/Cyanine5.5 Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09793I] |
Reactivity | Rat |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume]. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | PE/Cyanine5.5 |
PE/Cyanine5.5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 690 nm (e.g., a 690/50 nm bandpass filter). | |
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How to select the appropriate detection channel through the spectrogram?
CD8a is a 32 kD glycoprotein also known as T8, Lyt2, Ly-2, and CD8α. CD8a is a member of the immunoglobulin superfamily expressed on most thymocytes, subset of mature T cells, most NK cells, macrophages, and some activated CD4+ T cells (not resting). CD8a forms heterodimers with the CD8β chain (CD8b) on the surface of most thymocytes, while mature peripheral T lymphocytes express almost exclusively the CD8 αβ heterodimer. Intestinal intraepithelial lymphocytes express CD8a without CD8b. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck). |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
Product Name | Sizes | Price |
FITC Anti-Rat CD8a Antibody[OX-8] | 50Tests, 100Tests, 100Tests×2 | Check More |
PE Anti-Rat CD8a Antibody[OX-8] | 50Tests, 100Tests, 100Tests×2 | Check More |
APC Anti-Rat CD8a Antibody[OX-8] | 50Tests, 100Tests, 100Tests×2 | Check More |
PE/Cyanine7 Anti-Rat CD8a Antibody[OX-8] | 50Tests, 100Tests, 100Tests×2 | Check More |
PE/Cyanine5.5 Anti-Rat CD8a Antibody[OX-8] | 50Tests, 100Tests, 100Tests×2 | Check More |
Elab Fluor® 488 Anti-Rat CD8a Antibody[OX-8] | 50Tests, 100Tests, 100Tests×2 | Check More |
FITC Anti-Rat CD8a Antibody[OX-8] | 25μg, 100μg | Check More |
PE Anti-Rat CD8a Antibody[OX-8] | 25μg, 100μg | Check More |
APC Anti-Rat CD8a Antibody[OX-8] | 25μg, 100μg | Check More |
PE/Cyanine7 Anti-Rat CD8a Antibody[OX-8] | 25μg, 100μg | Check More |
Elab Fluor® 488 Anti-Rat CD8a Antibody[OX-8] | 25μg, 100μg | Check More |
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