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PE/Cyanine5 Anti-Mouse CD3ε Antibody[145-2C11] E-AB-F1103UG

Uniprot : P22646

Cat.No.:E-AB-F1103UG
Applications: FCM
Isotype: Armenian Hamster IgG
Host: Armenian Hamster

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Antigen Details

Alternate Names	T-cell surface glycoprotein CD3 epsilon chain,CD3E,T-cell surface antigen T3/Leu-4 epsilon chain,CD3e,CD3E,T3E
Swissprot	P22646
References	1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss A, et al. 1994. Cell 76:263.

Product Details

Form	Liquid
Clone No.	145-2C11(See Other Available Formats)
Host	Armenian Hamster
Isotype	Armenian Hamster IgG
Isotype Control	[Product E-AB-F09853G]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10⁶ cells in 100 μL volume].
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PE/Cyanine5
	PE/Cyanine5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse CD3ε Antibody[145-2C11] E-AB-F11030	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse CD3ε Antibody[145-2C11] E-AB-F1103A	25μg, 100μg	Check More
Biotin Anti-Mouse CD3ε Antibody[145-2C11] E-AB-F1103B	25μg, 100μg	Check More
FITC Anti-Mouse CD3ε Antibody[145-2C11] E-AB-F1103C	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD3ε Antibody[145-2C11] E-AB-F1103D	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD3ε Antibody[145-2C11] E-AB-F1103E	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD3ε Antibody[145-2C11] E-AB-F1103G	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD3ε Antibody[145-2C11] E-AB-F1103H	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD3ε Antibody[145-2C11] E-AB-F1103I	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD3ε Antibody[145-2C11] E-AB-F1103J	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD3ε Antibody[145-2C11]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD3ε Antibody[145-2C11]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD3ε Antibody[145-2C11]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD3ε Antibody[145-2C11] E-AB-F1103UC	25μg, 100μg	Check More
PE Anti-Mouse CD3ε Antibody[145-2C11] E-AB-F1103UD	25μg, 100μg	Check More
APC Anti-Mouse CD3ε Antibody[145-2C11] E-AB-F1103UE	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD3ε Antibody[145-2C11] E-AB-F1103UH	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD3ε Antibody[145-2C11] E-AB-F1103UI	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse CD3ε Antibody[145-2C11] E-AB-F1103UJ	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD3ε Antibody[145-2C11]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD3ε Antibody[145-2C11]	25μg, 100μg	Check More
Elab Fluor^® Violet 450 Anti-Mouse CD3ε Antibody[145-2C11]	25μg, 100μg	Check More

Citations

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