Alternate Names	Intercellular adhesion molecule 1,Icam1,MALA-2,MyD10,CD54,Icam-1
Swissprot	P13597
References	1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Springer TA. 1990. Nature 346:425. 3. Rothlein R, et al. 1986. J. Immunol. 137:1270.

Product Details

Form	Liquid
Clone No.	YN1/1.7.4
Host	Rat
Isotype	Rat IgG2b, κ
Isotype Control	PE/Cyanine5 Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09843G]
Reactivity	Mouse
Application	FCM
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10⁶ cells in 100 μL volume].
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PE/Cyanine5
	PE/Cyanine5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD54 is a 90 kD immunoglobulin superfamily member also known as ICAM-1 and Ly-47. It is expressed on activated endothelial cells, high endothelial venules (HEV), T and B cells, monocytes/ macrophages, granulocytes, and dendritic cells. CD54 is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. CD54 is also a receptor for rhinovirus. The YN1/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse CD54 Antibody[YN1/1.7.4]	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse CD54 Antibody[YN1/1.7.4]	25μg, 100μg	Check More
Biotin Anti-Mouse CD54 Antibody[YN1/1.7.4]	25μg, 100μg	Check More
FITC Anti-Mouse CD54 Antibody[YN1/1.7.4]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse CD54 Antibody[YN1/1.7.4]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse CD54 Antibody[YN1/1.7.4]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Mouse CD54 Antibody[YN1/1.7.4]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD54 Antibody[YN1/1.7.4]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse CD54 Antibody[YN1/1.7.4]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse CD54 Antibody[YN1/1.7.4]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD54 Antibody[YN1/1.7.4]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse CD54 Antibody[YN1/1.7.4]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD54 Antibody[YN1/1.7.4]	25μg, 100μg	Check More
PE Anti-Mouse CD54 Antibody[YN1/1.7.4]	25μg, 100μg	Check More
APC Anti-Mouse CD54 Antibody[YN1/1.7.4]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD54 Antibody[YN1/1.7.4]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse CD54 Antibody[YN1/1.7.4]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse CD54 Antibody[YN1/1.7.4]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD54 Antibody[YN1/1.7.4]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse CD54 Antibody[YN1/1.7.4]	25μg, 100μg	Check More

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