Alternate Names	Adhesion G protein-coupled receptor E1,Adgre1,Cell surface glycoprotein F4/80,EGF-like module receptor 1,Adgre1,Emr1, Gpf480
Swissprot	Q61549
References	1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805. 2. Hume DA, et al. 1983. J. Exp. Med. 158:1522. 3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801. 4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486. 5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Product Details

Form	Liquid
Clone No.	CI:A3-1(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2b, κ
Isotype Control	PE/Cyanine5 Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09842G]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PE/Cyanine5
	PE/Cyanine5 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 670 nm (e.g., a 690/50 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse F4/80 Antibody[CI:A3-1]	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More
Biotin Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More
FITC Anti-Mouse F4/80 Antibody[CI:A3-1]	50Tests, 100Tests, 100Tests×2	Check More
PE Anti-Mouse F4/80 Antibody[CI:A3-1]	50Tests, 100Tests, 100Tests×2	Check More
APC Anti-Mouse F4/80 Antibody[CI:A3-1]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse F4/80 Antibody[CI:A3-1]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5.5 Anti-Mouse F4/80 Antibody[CI:A3-1]	50Tests, 100Tests, 100Tests×2	Check More
PerCP/Cyanine5.5 Anti-Mouse F4/80 Antibody[CI:A3-1]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse F4/80 Antibody[CI:A3-1]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 647 Anti-Mouse F4/80 Antibody[CI:A3-1]	50Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Mouse F4/80 Antibody[CI:A3-1]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Violet 450 Anti-Mouse F4/80 Antibody[CI:A3-1]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® Red 780 Anti-Mouse F4/80 Antibody[CI:A3-1]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More
PE Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More
APC Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More
PE/Cyanine5 Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More
PE/Cyanine5.5 Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More
PerCP/Cyanine5.5 Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More
Elab Fluor^® 647 Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More
PE/Elab Fluor^® 594 Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More
Elab Fluor^® Violet 450 Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More
Elab Fluor^® Red 780 Anti-Mouse F4/80 Antibody[CI:A3-1]	25μg, 100μg	Check More

Citations

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