PE/Cyanine7 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] E-AB-F1131H

Uniprot : Q02242
  • Host: Rat
  • Applications: FCM
  • Isotype: Rat IgG2a, κ

To Purchase E-AB-F1131H

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Price: $45
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Antigen Details

Alternate Names PD-1, Programmed Death-1
Swissprot Q02242
References
1. Nishimura H, et al. 2001. Science 291:319
2. Agata Y, et al. 1996. Int. Immunol. 8:765
3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706
4. Barber DL, et al. 2006. Nature 439:682
5. Keir ME, et al. 2005. J. Immunol. 175:7372
6. Koehn BH. et al. 2015.

Product Details

Form Liquid
Clone No. 29F.1A12(See Other Available Formats)
Host Rat
Isotype Rat IgG2a, κ
Isotype Control PE/Cyanine7 Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09832H]
Reactivity Mouse
Application FCM (See the Application Description)
Storage Buffer PBS with 0.05% Proclin300, 1% BSA
Recommended Use Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 μL staining volume or per 100 μL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8℃ and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE/Cyanine7

Background

CD279, also known as programmed death-1 (PD-1), is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is expressed on activated splenic T and B cells and thymocytes. It is induced on activated myeloid cells as well. PD-1 is involved in lymphocyte clonal selection and peripheral tolerance through binding its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2). It has been reported that PD-1 and PD-L1 interactions are critical to positive selection and play a role in shaping the T cell repertoire. PD-L1 negative costimulation is essential for prolonged survival of intratesticular islet allografts.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Cat.No. Product Name Sizes
E-AB-F11310 AF/LE Purified Anti-Mouse CD279/PD-1 Antibody[29F.1A12] E-AB-F11310 50 μg, 500 μg, 1 mg
E-AB-F1131A Purified Anti-Mouse CD279/PD-1 Antibody[29F.1A12] E-AB-F1131A 50 μg, 100 μg
E-AB-F1131B Biotin Anti-Mouse CD279/PD-1 Antibody[29F.1A12] E-AB-F1131B 25 μg, 100 μg
E-AB-F1131C FITC Anti-Mouse CD279/PD-1 Antibody[29F.1A12] E-AB-F1131C 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1131D PE Anti-Mouse CD279/PD-1 Antibody[29F.1A12] E-AB-F1131D 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1131E APC Anti-Mouse CD279/PD-1 Antibody[29F.1A12] E-AB-F1131E 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1131H PE/Cyanine7 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] E-AB-F1131H 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1131I PE/Cyanine5.5 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] E-AB-F1131I 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1131J PerCP/Cyanine5.5 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] E-AB-F1131J 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1131M AF647 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] E-AB-F1131M 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1131UC FITC Anti-Mouse CD279/PD-1 Antibody[29F.1A12] E-AB-F1131UC 25μg, 50μg, 100μg
E-AB-F1131UD PE Anti-Mouse CD279/PD-1 Antibody[29F.1A12] E-AB-F1131UD 25μg, 50μg, 100μg
E-AB-F1131UE APC Anti-Mouse CD279/PD-1 Antibody[29F.1A12] E-AB-F1131UE 25μg, 50μg, 100μg
E-AB-F1131UH PE/Cyanine7 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] E-AB-F1131UH 25μg, 50μg, 100μg
E-AB-F1131UI PE/Cyanine5.5 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] E-AB-F1131UI 25μg, 50μg, 100μg
E-AB-F1131UJ PerCP/Cyanine5.5 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] E-AB-F1131UJ 25μg, 50μg, 100μg
E-AB-F1131UM AF647 Anti-Mouse CD279/PD-1 Antibody[29F.1A12] E-AB-F1131UM 25μg, 50μg, 100μg

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