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Pepsin Activity Assay Kit

Cat.No.:E-BC-K844-M
Detection instrument: Microplate reader (650-670 nm, optimum wavelength: 660 nm)

Detection method: Colorimetric method

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96T

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Detection principle

Pepsin can catalyze substrate hydrolysis, and the hydrolysate reacts with the chromogenic agent under alkaline conditions to form a blue substance. The blue substance has a maximum absorption peak at 660 nm, and the color depth is proportional to the activity of pepsin. The enzyme activity can be calculated by OD value

Performance characteristics

Sample type	gastric, intestinal tissue
Sensitivity	0.63 U/mL
Detection range	0.63-94.98 U/mL
Detection method	Colorimetric method
Assay type	Enzyme Activity
Precision	Average inter-assay CV: 4.8-5.6%Average intra-assay CV: 1.3-2.2%
Storage	-20℃
Valid period	12 months

Ralated product

Cat Number	Product Name
E-BC-K843-M	Trypsin Activity Assay Kit

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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