Pepsin Activity Assay Kit (E-BC-K844-M)
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For research use only.
| Detection Principle | Pepsin can catalyze substrate hydrolysis, and the hydrolysate reacts with the chromogenic agent under alkaline conditions to form a blue substance. The blue substance has a maximum absorption peak at 660 nm, and the color depth is proportional to the activity of pepsin. The enzyme activity can be calculated by OD value. |
| Sample Type | Gastric,Intestinal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (650-670 nm, optimum wavelength: 660 nm) |
| Research Area | Amino Acids And Proteins |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.63 U/mL |
| Detection Range | 0.63-94.98 U/mL |
| Precision | inter-assay CV: 4.8-5.6% | intra-assay CV: 1.3-2.2% |
| Sample Volume | 80 μL(tissue homogenate) |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 20% Rat gastric tissue homogenate | 1 |
| 20% Mouse gastric tissue homogenate | 1 |
| 20% Rat intestinal tissue homogenate | 1 |
| 20% Mouse intestinal tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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