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PerCP Anti-Human CD16 Antibody[3G8] E-AB-F1236F

Uniprot : P33681
  • Cat.No.:E-AB-F1236F

  • Reactivity: Human

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Antigen Details

Alternate Names T-lymphocyte activation antigen CD16,B7-1, B7, BB1
Swissprot P33681

Product Details

Form Liquid
Clone No. 3G8(See Other Available Formats)
Host Mouse
Isotype Mouse IgG1, κ
Isotype Control PerCP Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792F]
Reactivity Human
Application FCM (See the Application Description)
Storage Buffer PBS with 0.05% Proclin300, 1% BSA
Recommended Use Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 μL staining volume or per 100 μL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8℃ and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PerCP

Background

CD16 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD16 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD16 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD16 appears to be expressed later in the immune response than CD86. CD16 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Cat.No. Product Name Sizes
E-AB-F1236C FITC Anti-Human CD16 Antibody[3G8] E-AB-F1236C 20Tests, 50Tests, 100Tests, 200Tests
E-AB-F1236D PE Anti-Human CD16 Antibody[3G8] E-AB-F1236D 20Tests, 50Tests, 100Tests, 200Tests
E-AB-F1236E APC Anti-Human CD16 Antibody[3G8] E-AB-F1236E 20Tests, 50Tests, 100Tests, 200Tests
E-AB-F1236F PerCP Anti-Human CD16 Antibody[3G8] E-AB-F1236F 20Tests, 50Tests, 100Tests, 200Tests
E-AB-F1236J PerCP/Cyanine5.5 Anti-Human CD16 Antibody[3G8] E-AB-F1236J 20Tests, 50Tests, 100Tests, 200Tests

Citations

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