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PerCP/Cyanine5.5 Anti-Mouse F4/80 Antibody[CI:A3-1]

  • Cat.No.:E-AB-F0995J

  • Applications: FCM
  • Isotype: Rat IgG2b, κ
  • Host: Rat

To Purchase E-AB-F0995J

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  • 50Tests
  • 100Tests
  • 100Tests×2
Price: $48
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Antigen Details

Alternate Names Adhesion G protein-coupled receptor E1,Adgre1,Cell surface glycoprotein F4/80,EGF-like module receptor 1,Adgre1,Emr1, Gpf480
Swissprot Q61549
References
1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Product Details

Form Liquid
Clone No. CI:A3-1
Host Rat
Isotype Rat IgG2b, κ
Isotype Control PerCP/Cyanine5.5 Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09842J]
Reactivity Mouse
Application FCM
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PerCP/Cyanine5.5
PerCP/Cyanine5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).


How to select the appropriate detection channel through the spectrogram?

Background

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Product Name Sizes Price
AF/LE Purified Anti-Mouse F4/80 Antibody[CI:A3-1] 50μg, 500μg, 1mg Check More
Purified Anti-Mouse F4/80 Antibody[CI:A3-1] 25μg, 100μg Check More
Biotin Anti-Mouse F4/80 Antibody[CI:A3-1] 25μg, 100μg Check More
FITC Anti-Mouse F4/80 Antibody[CI:A3-1] 50Tests, 100Tests, 100Tests×2 Check More
PE Anti-Mouse F4/80 Antibody[CI:A3-1] 50Tests, 100Tests, 100Tests×2 Check More
APC Anti-Mouse F4/80 Antibody[CI:A3-1] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5 Anti-Mouse F4/80 Antibody[CI:A3-1] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine7 Anti-Mouse F4/80 Antibody[CI:A3-1] 50Tests, 100Tests, 100Tests×2 Check More
PE/Cyanine5.5 Anti-Mouse F4/80 Antibody[CI:A3-1] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 488 Anti-Mouse F4/80 Antibody[CI:A3-1] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® 647 Anti-Mouse F4/80 Antibody[CI:A3-1] 50Tests, 100Tests, 100Tests×2 Check More
PE/Elab Fluor® 594 Anti-Mouse F4/80 Antibody[CI:A3-1] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Violet 450 Anti-Mouse F4/80 Antibody[CI:A3-1] 50Tests, 100Tests, 100Tests×2 Check More
Elab Fluor® Red 780 Anti-Mouse F4/80 Antibody[CI:A3-1] 50Tests, 100Tests, 100Tests×2 Check More
FITC Anti-Mouse F4/80 Antibody[CI:A3-1] 25μg, 100μg Check More
PE Anti-Mouse F4/80 Antibody[CI:A3-1] 25μg, 100μg Check More
APC Anti-Mouse F4/80 Antibody[CI:A3-1] 25μg, 100μg Check More
PE/Cyanine5 Anti-Mouse F4/80 Antibody[CI:A3-1] 25μg, 100μg Check More
PE/Cyanine7 Anti-Mouse F4/80 Antibody[CI:A3-1] 25μg, 100μg Check More
PE/Cyanine5.5 Anti-Mouse F4/80 Antibody[CI:A3-1] 25μg, 100μg Check More
PerCP/Cyanine5.5 Anti-Mouse F4/80 Antibody[CI:A3-1] 25μg, 100μg Check More
Elab Fluor® 488 Anti-Mouse F4/80 Antibody[CI:A3-1] 25μg, 100μg Check More
Elab Fluor® 647 Anti-Mouse F4/80 Antibody[CI:A3-1] 25μg, 100μg Check More
PE/Elab Fluor® 594 Anti-Mouse F4/80 Antibody[CI:A3-1] 25μg, 100μg Check More
Elab Fluor® Violet 450 Anti-Mouse F4/80 Antibody[CI:A3-1] 25μg, 100μg Check More
Elab Fluor® Red 780 Anti-Mouse F4/80 Antibody[CI:A3-1] 25μg, 100μg Check More

Citations

  1. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208
    Hepatocyte-Derived Prostaglandin E2-Modulated Macrophage M1-Type Polarization via mTOR-NPC1 Axis-Regulated Cholesterol Transport from Lysosomes to the Endoplasmic Reticulum in Hepatitis B Virus x Protein-Related Nonalcoholic Steatohepatitis

    DOI: 10.3390/ijms231911660

    PMID: 36232960

    Sample: Kupffer Cells
  2. Biomaterials Science (2021) IF: 6.843
    Supramolecular copolymer modified statin-loaded discoidal rHDLs for atherosclerotic anti-inflammatory therapy by cholesterol efflux and M2 macrophage polarization

    DOI: 10.1039/D1BM00610J

    PMID: 34346410

    Sample: Raw 264.7 Cell
  3. Advanced Science (2023) IF: 17.521
    Nanomedicines Reprogram Synovial Macrophages by Scavenging Nitric Oxide and Silencing CA9 in Progressive Osteoarthritis

    DOI: 10.1002/advs.202207490

    PMID: 36748885

    Sample: Raw264.7 Cell
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