PGE2(Prostaglandin E2) ELISA Kit

    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    <
    >
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience

      Catalog number:E-EL-0034

      Synonyms:PG-E2, Dinoprostone

      Size:
      • 96T
      • 24T
      Qty:
      - +
      Price: $495

      Reactivity: Universal

      Detection Range: 31.25~2000 pg/mL

      Sensitivity: 18.75 pg/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Intended use

      This ELISA kit applies to the in vitro quantitative determination of  PGE2 concentrations in serum, plasma and other biological fluids.

      Test principle

      This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with PGE2. During the reaction,  PGE2 in the sample or standard competes with a fixed amount of PGE2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to  PGE2. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of  PGE2 in the samples is then determined by comparing the OD of the samples to the standard curve.

      Assay type Competitive
      Format 96T
      Assay time 2.0h
      Reactivity Universal
      Detection Method Colormetric
      Detection Range 31.25—2000 pg/mL
      Sensitivity 18.75 pg/mL
      Sample Volume 50μL
      Sample Type Serum, plasma and other biological fluids

      Specificity

      This kit recognizes PGE2 in samples. No significant cross-reactivity or interference between PGE2 and analogues was observed.

      Typical data

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      Concentration(pg/mL) O.D Average
      2000 0.381
      0.393
      0.387
      1000 0.489
      0.517
      0.503
      500 0.702
      0.696
      0.699
      250 0.974
      1.002
      0.988
      125 1.341
      1.331
      1.336
      62.5 1.674
      1.662
      1.668
      31.25 1.913
      1.925
      1.919
      0 2.261
      2.269
      2.265

      Precision

      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level PGE2 were tested 20 times on one plate, respectively.
      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level PGE2 were tested on 3 different plates, 20 replicates in each plate.

        Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean (pg/mL) 101.30 236.80 779.30 96.00 259.90 728.60
      Standard deviation 6.60 11.60 42.10 4.90 13.80 31.30
      C V (%) 6.52 4.90 5.40 5.10 5.31 4.30

      Recovery

      The recovery of  PGE2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum (n=5) 92-109 99
      EDTA plasma (n=5) 87-99 94
      Cell culture media (n=5) 86-96 91

      Linearity

      Samples were spiked with high concentrations of PGE2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

          Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
      1:2 Range (%) 95-110 91-104 89-101
      Average (%) 103 98 96
      1:4 Range (%) 85-95 89-101 101-117
      Average (%) 90 95 107
      1:8 Range (%) 87-100 92-104 96-111
      Average (%) 92 97 102
      1:16 Range (%) 88-104 93-105 99-114
      Average (%) 95 98 106

      Kit components & Storage

      An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

      Item Specifications Storage
      Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20℃, 6 months
      Reference Standard 2 vials
      Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
      Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20℃(shading light), 6 months
      Reference Standard & Sample Diluent 1 vial, 20 mL 4℃, 6 months
      Biotinylated Detection Ab Diluent 1 vial, 14 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Substrate Reagent 1 vial, 10 mL 4℃(shading light)
      Stop Solution 1 vial, 10 mL 4℃
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      • Microplate reader with 450 nm wavelength filter
      • High-precision transfer pipette, EP tubes and disposable pipette tips
      • Incubator capable of maintaining 37℃
      • Deionized or distilled water
      • Absorbent paper
      • Loading slot for Wash Buffer

      Assay Procedure

      elisa assay procedure 1

      1.Add 50 μL standard or sample to each well.

      elisa assay procedure 2

      2.Immediately add 50 μL Biotinylated Detection Ab to each well.

      elisa assay procedure 3

      3.Incubate for 45 min at 37℃. Aspirate and wash 3 times.

      elisa assay procedure 4

      4.Add 100 μL HRP Conjugate to each well. Incubate for 30 min at 37℃. Aspirate and wash 5 times.

      elisa assay procedure 5

      5.Add 90 μL Substrate Reagent. Incubate for 15 min at 37℃.

      elisa assay procedure 6

      6.Add 50 μL Stop Solution.

      elisa assay procedure 7

      7.Read at 450 nm immediately. Calculation of results.

      Citations

      1. Publication: Zhu S Y, Li J, Bing Y T, et al. Diet Induced Hyperhomocysteinemia Increases Intestinal Inflammation In An Animal Model Of Colitis[J]. Journal of Crohn's & Colitis, 2015.
        Sample Type: Plasma
      2. Publication: Zhou F, Zhang W, Zhou J, et al. Involvement Of Endoplasmic Reticulum Stress In Formalin-Induced Pain Is Attenuated By 4-Phenylbutyric Acid[J]. Journal of Pain Research, 2017, 10: 653-662.
        Sample Type: Spinal cord
      3. Publication: Zhang Z, Li X F, Huang H, et al. Cross-Coupling Effects Of Silencing Of Cyclooxygenase-2 (COX-2)/Aggrecanase-1 And Over-Expressed Insulin-Like Growth Factor 1 (IGF-1) In An Osteoarthritis Animal Model[J]. Medical Science Monitor: International Medical Journal of Experimental And Clinical Research, 2017, 23: 5302-5310.
        Sample Type: synovial fluid
      4. Publication: DIK B, SONMEZ G, FAKI H E, et al. Sulfasalazine treatment can cause a positive effect on LPS-induced endotoxic rats[J]. Experimental animals, 2018: 18-0029.
        Sample Type: Rat
      5. Publication: Lu H , Yao H , Zou R , et al. Galangin Suppresses Renal Inflammation via the Inhibition of NF-κB, PI3K/AKT and NLRP3 in Uric Acid Treated NRK-52E Tubular Epithelial Cells[J]. BioMed Research International, 2019.
        Sample Type: Cell supernatants
      Show AllShorten
      • Show all (0)
      • Reviews (0)
      • Q&A (0)
      ... Show All Show Less
      MSDS for ELISA

      Browsing History


        People Also Bought