Plant Flavonoids Colorimetric Assay Kit (E-BC-K284-S)
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For research use only.
| Detection Principle | In alkaline nitrite solution, flavonoids form red complex with aluminum ion. The flavonoid content of the sample can be calculated by measuring the absorptivity of the sample extract at 510 nm. |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (510 nm) |
| Research Area | Plant Physiology Series |
| Other Reagents Required | 60% Ethanol |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.315 μg/mL |
| Detection Range | 0.315-150 μg/mL |
| Precision | inter-assay CV: 2.2% | intra-assay CV: 1.9% |
| Sample Volume | 0.54 mL(tissue homogenate) |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Camphor leaves tissue homogenization | 8-15 |
| Carrot tissue homogenization | 2-5 |
| Green pepper tissue homogenization | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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